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Tumor Necrosis Factor-Alpha (tnfa) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546103

Tumor necrosis factor alpha (TNF-á), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-á occurs as a secreted,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Tumor Necrosis Factor-alpha (tnfa), Active Protein
Full Product Name
Recombinant Human Tumor Necrosis Factor-alpha (uHu TNF-á)
Product Gene Name
Product Synonym Names
Tumor Necrosis Factor-alpha; human TNF-alpha; Human Tumor Necrosis Factor-alpha; Tumor Necrosis Factor-alpha; human TNF-a
Host
E Coli
Form/Format
Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.0.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
10ug/50ug/1000ìg
Preparation and Storage
This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeatedfreeze/thaw cycles.
Chromosome Location
chromosome: Un
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Molecular Weight Information
Approximately 17.5 kDa protein containing 158 amino acid residues.
Biological Activity
Fully biologically active when compared to standard. The Specific Activity is >=2.0 *107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Endotoxin Level
Less than 1EU/ug of rHuTNF-alpha as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of tnfa active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for tnfa purchase

MBS546103 is an active protein targeting tnfa. Also known as Recombinant Human Tumor Necrosis Factor-alpha (uHu TNF-á). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for tnfa. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor alpha
NCBI Official Symbol
tnfa
NCBI Protein Information
tumor necrosis factor alpha

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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