Human CX3CL1 Active Protein – Cat# MBS546141

Fractalkine (CX3CL1) Human Active Protein

Name: CX3CL1 Active Protein; Fractalkine; Human; MyBioSource
Brand: MyBioSource
SKU: MBS546141
Price: 205 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546141

Fractalkine, also named neurotactin, is a novel chemokine recently identified through bioinformatics. Fractalkine has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Fractalkine (CX3CL1), Active Protein

Full Product Name

Recombinant Human Fractalkine (rHu Fractalkine/CX3CL1)

Product Gene Name

CX3CL1 active protein

Product Synonym Names

human Fractalkine (CX3CL1); Human Fractalkine/CX3CL1

Host

E Coli

Form/Format

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.
Physical Appearance: Sterile FilteredWhite lyophilized (freeze-dried) powder

Purity/Purification

>97% by SDS-PAGE and HPLC analyses

Preparation and Storage

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles

Molecular Weight

42,203 Da

Chromosome Location

Chromosome: 16; NC_000016.9 (57406414..57418956). Location: 16q13

OMIM

601880

3D Structure

ModBase 3D Structure for P78423

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Molecular Weight Information

8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.

Biological Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 5.0-10.0 ng/ml.

Endotoxin Level

Less than 1EU/mg of rHuFractalkine/CX3CL1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of CX3CL1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CX3CL1 purchase

MBS546141 is an active protein targeting CX3CL1. Also known as Recombinant Human Fractalkine (rHu Fractalkine/CX3CL1). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CX3CL1. It may not necessarily be applicable to this product.

NCBI GI #

4506857

NCBI GeneID

6376

NCBI Accession #

NP_002987.1

UniProt Primary Accession #

P78423

UniProt Secondary Accession #

O00672

UniProt Related Accession #

P78423

GenBank Nucleotide #

NM_002996.3

NCBI Official Full Name

fractalkine

NCBI Official Synonym Full Names

chemokine (C-X3-C motif) ligand 1

NCBI Official Symbol

CX3CL1

NCBI Official Synonym Symbols

NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

UniProt Protein Name

Fractalkine

UniProt Synonym Protein Names

C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1

Protein Family

Fractalkine

UniProt Gene Name

CX3CL1

UniProt Synonym Gene Names

FKN; NTT; SCYD1

UniProt Entry Name

X3CL1_HUMAN

NCBI Protein Information

fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)

UniProt Comments for CX3CL1

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; plasma membrane; extracellular region; integral to membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: positive regulation of transforming growth factor-beta1 production; neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; defense response; chemotaxis; angiogenesis involved in wound healing; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response

Research Articles on CX3CL1

1. fat mass alone is not responsible for elevation of fractalkine seen in obese individuals

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CX3CL1 active protein

Reference Product	PubMed Publications
CX3CR1 active protein	>413 publications with CX3CL1 and CX3CR1
CCR2 active protein	>49 publications with CX3CL1 and CCR2
CXCR4 active protein	>32 publications with CX3CL1 and CXCR4
CCR5 active protein	>32 publications with CX3CL1 and CCR5
CXCR3 active protein	>23 publications with CX3CL1 and CXCR3
CCR1 active protein	>17 publications with CX3CL1 and CCR1
CCR3 active protein	>11 publications with CX3CL1 and CCR3
CXCR6 active protein	>9 publications with CX3CL1 and CXCR6

Pathways associated with CX3CL1 active protein

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with CX3CL1 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>280 publications with CX3CL1 and Inflammation
Necrosis Antibodies	>155 publications with CX3CL1 and Necrosis
Cardiovascular Diseases Antibodies	>123 publications with CX3CL1 and Cardiovascular Diseases
Brain Diseases Antibodies	>79 publications with CX3CL1 and Brain Diseases
Atherosclerosis Antibodies	>76 publications with CX3CL1 and Atherosclerosis
Pain Antibodies	>49 publications with CX3CL1 and Pain
Kidney Diseases Antibodies	>45 publications with CX3CL1 and Kidney Diseases
Heart Diseases Antibodies	>39 publications with CX3CL1 and Heart Diseases
Liver Diseases Antibodies	>35 publications with CX3CL1 and Liver Diseases
Myocardial Ischemia Antibodies	>34 publications with CX3CL1 and Myocardial Ischemia

Organs/Tissues associated with CX3CL1 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>152 publications with CX3CL1 and Brain
Lung Antibodies	>76 publications with CX3CL1 and Lung
Muscle Antibodies	>74 publications with CX3CL1 and Muscle
Kidney Antibodies	>72 publications with CX3CL1 and Kidney
Liver Antibodies	>53 publications with CX3CL1 and Liver
Heart Antibodies	>50 publications with CX3CL1 and Heart
Nerve Antibodies	>43 publications with CX3CL1 and Nerve
Skin Antibodies	>36 publications with CX3CL1 and Skin
Intestine Antibodies	>31 publications with CX3CL1 and Intestine
Eye Antibodies	>21 publications with CX3CL1 and Eye