Human ENA-78 Active Protein – Cat# MBS546155

ENA-78 Human Active Protein

Name: ENA-78 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS546155
Price: 205 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546155

Epithelial cell-derived neutrophil-activating peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines that has the Glu-Leu-Arg (ELR) motif preceding the CXC motif. Similar to other ELR containing CXC chemokines, ENA-78 is a potent neutrophil… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ENA-78, Active Protein

Full Product Name

Recombinant Human ENA-78(5-78a.a.) (rHu ENA-78(CXCL5))

Product Gene Name

ENA-78 active protein

Product Synonym Names

ENA-78(5-78a.a.; human ENA-78/CXCL5(5-78a.a.); Human ENA-78/CXCL5(5-78a.a.)

Host

E Coli

Form/Format

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl
Physical Appearance: Sterile FilteredWhite lyophilized (freeze-dried) powder.

Purity/Purification

>97% by SDS-PAGE and HPLC analyses

Preparation and Storage

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Molecular Weight

11,972 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (74861359..74864446, complement). Location: 4q13.3

OMIM

600324

3D Structure

ModBase 3D Structure for P42830

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Molecular Weight Information

8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Biological Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 5.0-10.0 ng/ml

Endotoxin Level

Less than 1EU/mg of rHuENA-78/CXCL5 as determined by LAL method

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions

Other Notes

Small volumes of ENA-78 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENA-78 purchase

MBS546155 is an active protein targeting ENA-78. Also known as Recombinant Human ENA-78(5-78a.a.) (rHu ENA-78(CXCL5)). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ENA-78. It may not necessarily be applicable to this product.

NCBI GI #

471243

NCBI GeneID

6374

NCBI Accession #

CAA55355.1

UniProt Primary Accession #

P42830

UniProt Secondary Accession #

Q96QE1

UniProt Related Accession #

P42830

NCBI Official Full Name

ENA-78

NCBI Official Synonym Full Names

chemokine (C-X-C motif) ligand 5

NCBI Official Symbol

CXCL5

NCBI Official Synonym Symbols

SCYB5; ENA-78

UniProt Protein Name

C-X-C motif chemokine 5

UniProt Synonym Protein Names

ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5

UniProt Gene Name

CXCL5

UniProt Synonym Gene Names

ENA78; SCYB5

UniProt Entry Name

CXCL5_HUMAN

NCBI Protein Information

C-X-C motif chemokine 5; neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; epithelial-derived neutrophil-activating protein 78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)

NCBI Summary for ENA-78

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]

UniProt Comments for ENA-78

CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; signal transduction; inflammatory response; chemotaxis

Research Articles on ENA-78

1. Liver cancer cells with high metastatic potential have a higher expression of CXCL5. Exogenous CXCL5 can increase the proliferation, migration and invasion of liver cancer cells with low metastatic potential.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ENA-78 active protein

Reference Product	PubMed Publications
CXCR2 active protein	>69 publications with ENA-78 and CXCR2
CCL5 active protein	>66 publications with ENA-78 and CCL5
CCL20 active protein	>34 publications with ENA-78 and CCL20
CXCR1 active protein	>25 publications with ENA-78 and CXCR1
CXCL11 active protein	>16 publications with ENA-78 and CXCL11
CXCL16 active protein	>8 publications with ENA-78 and CXCL16
CCR3 active protein	>8 publications with ENA-78 and CCR3
CCR1 active protein	>7 publications with ENA-78 and CCR1
CCL19 active protein	>6 publications with ENA-78 and CCL19
CCR5 active protein	>6 publications with ENA-78 and CCR5

Pathways associated with ENA-78 active protein

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram

Diseases associated with ENA-78 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>189 publications with ENA-78 and Inflammation
Neoplasms Antibodies	>104 publications with ENA-78 and Neoplasms
Necrosis Antibodies	>87 publications with ENA-78 and Necrosis
Respiratory Tract Diseases Antibodies	>82 publications with ENA-78 and Respiratory Tract Diseases
Lung Diseases Antibodies	>73 publications with ENA-78 and Lung Diseases
Cardiovascular Diseases Antibodies	>38 publications with ENA-78 and Cardiovascular Diseases
Fibrosis Antibodies	>25 publications with ENA-78 and Fibrosis
Lung Neoplasms Antibodies	>22 publications with ENA-78 and Lung Neoplasms
Pneumonia Antibodies	>21 publications with ENA-78 and Pneumonia
Liver Diseases Antibodies	>17 publications with ENA-78 and Liver Diseases

Organs/Tissues associated with ENA-78 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>256 publications with ENA-78 and Blood
Lung Antibodies	>106 publications with ENA-78 and Lung
Vascular Antibodies	>85 publications with ENA-78 and Vascular
Intestine Antibodies	>30 publications with ENA-78 and Intestine
Skin Antibodies	>24 publications with ENA-78 and Skin
Brain Antibodies	>15 publications with ENA-78 and Brain
Connective Tissue Antibodies	>14 publications with ENA-78 and Connective Tissue
Uterus Antibodies	>13 publications with ENA-78 and Uterus
Kidney Antibodies	>13 publications with ENA-78 and Kidney
Embryonic Tissue Antibodies	>13 publications with ENA-78 and Embryonic Tissue