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Fibroblast Growth Factor-Basic Bovine Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546160

bFGF is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Fibroblast Growth Factor-basic, Active Protein
Full Product Name
Recombinant Bovine Fibroblast Growth Factor-basic (rBoFGF-2)
Product Synonym Names
Fibroblast Growth Factor-basic; bovine FGF-basic; Bovine Fibroblast Growth Factor-basic
Host
E Coli
Form/Format
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4, containing 4% mannitol.
Purity/Purification
>95% by SDS-PAGE and HPLC analyses.
Preparation and Storage
This lyophilized preparation is stable for several weeks at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Molecular Weight Information
Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 cells is < 1.0ng/ml, corresponding to a specific activity of 1.0x106Units/mg
Endotoxin Level
Less than 1EU/mg of rBoFGF-2 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of Fibroblast Growth Factor-basic active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fibroblast Growth Factor-basic purchase

MBS546160 is an active protein targeting Fibroblast Growth Factor-basic. Also known as Recombinant Bovine Fibroblast Growth Factor-basic (rBoFGF-2). Host: E Coli.
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