Human IGF-BP3 Active Protein – Cat# MBS546162

IGF-BP3 Human Active Protein

Name: IGF-BP3 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS546162
Price: 205 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546162

IGF-BP3 is a 30 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in á-granules of platelets. In addition to its ability to modulate the activity of IGF-I and IGF-II,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

IGF-BP3, Active Protein

Full Product Name

Recombinant Human IGF-BP3 (rHu IGF-BP3)

Product Gene Name

IGF-BP3 active protein

Product Synonym Names

human IGF-BP3; Human Insulin-like Growth Factor-Binding Protein 3(rHuIGF-BP3)

Sequence

GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPA PPAPGNASES EEDRSAGEVE SPSVSSTHRV SDPKFHPLHS KIIIIKKGHA KDSQRYKVDY ESQSTDTQNF SSESKRETEY GPCRREMEDT LNHLKFLNVL SPRGVHIPNC DKKGFYKKKQ CRPSKGRKRG FCWCVDKYGQ PLPGYTTKGK EDVHCYS MQSK

Host

E Coli

Form/Format

Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.

Purity/Purification

>98% by SDS-PAGE and HPLC analyses.

Preparation and Storage

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Molecular Weight

28.8 kDa protein consisting of 264 amino acids residues.

Chromosome Location

Chromosome: 14; NC_005113.3 (81091987..81099675, complement). Location: 14q21

3D Structure

ModBase 3D Structure for P15473

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Endotoxin Level

Less than 1EU/ug of rHuIGF-BP3 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

The ED₅₀ was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED₅₀ for this effect is < 0.2 ug/ml in presence of 15 ng/ml of human IGF-II.

Other Notes

Small volumes of IGF-BP3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGF-BP3 purchase

MBS546162 is an active protein targeting IGF-BP3. Also known as Recombinant Human IGF-BP3 (rHu IGF-BP3). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IGF-BP3. It may not necessarily be applicable to this product.

NCBI GI #

298231182

NCBI GeneID

24484

NCBI Accession #

NP_036720.2

UniProt Primary Accession #

P15473

UniProt Related Accession #

P15473

GenBank Nucleotide #

NM_012588.2

NCBI Official Full Name

insulin-like growth factor-binding protein 3

NCBI Official Synonym Full Names

insulin-like growth factor binding protein 3

NCBI Official Symbol

Igfbp3

NCBI Official Synonym Symbols

IGF-BP3

UniProt Protein Name

Insulin-like growth factor-binding protein 3

UniProt Gene Name

Igfbp3

UniProt Synonym Gene Names

Igfbp-3; IBP-3; IGF-binding protein 3; IGFBP-3

UniProt Entry Name

IBP3_RAT

NCBI Protein Information

insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; insulin-like growth factor-binding protein (IGF-BP3)

NCBI Summary for IGF-BP3

an insulin growth factor (Igf) binding protein; involved in modulating IGF-I action [RGD, Feb 2006]

UniProt Comments for IGF-BP3

IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation

Cellular Component: extracellular space; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; osteoblast differentiation; negative regulation of cell proliferation; regulation of insulin-like growth factor receptor signaling pathway; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; regulation of growth; negative regulation of smooth muscle cell migration; regulation of cell growth; protein amino acid phosphorylation; positive regulation of myoblast differentiation

Research Articles on IGF-BP3

1. IGFBP-3 has novel protective effects on retinal and systemic vasculature and may be a therapeutic candidate for ocular complications such as diabetic retinopathy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IGF-BP3 active protein

Reference Product	PubMed Publications
Igf1 active protein	>182 publications with IGF-BP3 and Igf1
Igf2 active protein	>66 publications with IGF-BP3 and Igf2
Egfr active protein	>35 publications with IGF-BP3 and Egfr
Prl active protein	>19 publications with IGF-BP3 and Prl
Igfals active protein	>16 publications with IGF-BP3 and Igfals
Vegfa active protein	>12 publications with IGF-BP3 and Vegfa
Ctsg active protein	>1 publications with IGF-BP3 and Ctsg

Pathways associated with IGF-BP3 active protein

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Myometrial Relaxation And Contraction Pathways antibodies	Myometrial Relaxation And Contraction Pathways Diagram
Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies	Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
P53 Pathway antibodies	P53 Pathway Diagram
P53 Signal Pathway antibodies	P53 Signal Pathway Diagram
P53 Signaling Pathway antibodies	P53 Signaling Pathway Diagram
P53 Signaling Pathway antibodies	P53 Signaling Pathway Diagram

Diseases associated with IGF-BP3 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1212 publications with IGF-BP3 and Neoplasms
Breast Neoplasms Antibodies	>329 publications with IGF-BP3 and Breast Neoplasms
Prostatic Neoplasms Antibodies	>251 publications with IGF-BP3 and Prostatic Neoplasms
Cardiovascular Diseases Antibodies	>207 publications with IGF-BP3 and Cardiovascular Diseases
Adenocarcinoma Antibodies	>165 publications with IGF-BP3 and Adenocarcinoma
Colorectal Neoplasms Antibodies	>140 publications with IGF-BP3 and Colorectal Neoplasms
Liver Diseases Antibodies	>137 publications with IGF-BP3 and Liver Diseases
Kidney Diseases Antibodies	>119 publications with IGF-BP3 and Kidney Diseases
Inflammation Antibodies	>111 publications with IGF-BP3 and Inflammation
Necrosis Antibodies	>104 publications with IGF-BP3 and Necrosis

Organs/Tissues associated with IGF-BP3 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>395 publications with IGF-BP3 and Liver
Muscle Antibodies	>281 publications with IGF-BP3 and Muscle
Connective Tissue Antibodies	>209 publications with IGF-BP3 and Connective Tissue
Kidney Antibodies	>198 publications with IGF-BP3 and Kidney
Brain Antibodies	>192 publications with IGF-BP3 and Brain
Lung Antibodies	>169 publications with IGF-BP3 and Lung
Ovary Antibodies	>156 publications with IGF-BP3 and Ovary
Heart Antibodies	>123 publications with IGF-BP3 and Heart
Embryonic Tissue Antibodies	>75 publications with IGF-BP3 and Embryonic Tissue
Placenta Antibodies	>73 publications with IGF-BP3 and Placenta