Human GRO-gamma Active Protein – Cat# MBS546197

GRO-gamma Human Active Protein

Name: GRO-gamma Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS546197
Price: 290 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546197

The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

GRO-gamma, Active Protein

Full Product Name

Recombinant Human GRO-gamma (rHu GRO-gamma/CXCL3)

Product Gene Name

GRO-gamma active protein

Product Synonym Names

GRO-gamma; human GROgamma (CXCL3); Human GRO-gamma/CXCL3; GRO-gamma; human GROg (CXCL3)

Host

E Coli

Form/Format

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.
Physical Appearance: Sterile FilteredWhite lyophilized (freeze-dried) powder

Purity/Purification

>97% by SDS-PAGE and HPLC analyses.

Preparation and Storage

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Molecular Weight Information

7.8 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.

Biological Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract hCXCR2 transfected 293 cells using a concentration range of 10.0- 100.0 ng/ml

Endotoxin Level

Less than 1EU/mg of rHuGRO-gamma/CXCL3 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions

Other Notes

Small volumes of GRO-gamma active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRO-gamma purchase

MBS546197 is an active protein targeting GRO-gamma. Also known as Recombinant Human GRO-gamma (rHu GRO-gamma/CXCL3). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GRO-gamma. It may not necessarily be applicable to this product.

NCBI GI #

30023531

NCBI Accession #

AAP13105.1

NCBI Official Full Name

GRO-gamma

Protein Family

Growth-regulated protein

NCBI Summary for GRO-gamma

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]

UniProt Comments for GRO-gamma

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: neutrophil chemotaxis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; inflammatory response; regulation of cell proliferation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.