G-CSF Active Protein

Name: G-CSF Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS546277
Price: 320 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546277

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-a and IFN-y. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-a… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

G-CSF, Active Protein

Full Product Name

G-CSF, mouse

Product Gene Name

G-CSF active protein

Sequence

VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

Form/Format

Lyophilized from a 0.2 um filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCI, 0.01 % Tween-20.

Purity/Purification

> 98% by SDS-PAGE and HPLC analyses.

Preparation and Storage

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree Cto -70 degree C.
Avoid repeated freeze/thaw cycles.

OMIM

138970

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source

Escherichia coli

Molecular Weight Information

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.

Endotoxin

Less than 0.1 EU/ug of rMuG-CSF as determined by LAL method.

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10⁷ IU/mg

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20°C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of G-CSF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G-CSF purchase

MBS546277 is an active protein targeting G-CSF. Also known as G-CSF, mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for G-CSF. It may not necessarily be applicable to this product.

NCBI GI #

743523204

NCBI GeneID

1440

NCBI Accession #

AJC19277.1

UniProt Secondary Accession #

A8MXR7

UniProt Related Accession #

P09919

NCBI Official Full Name

granulocyte-colony stimulating factor, partial

NCBI Official Synonym Full Names

colony stimulating factor 3

NCBI Official Symbol

CSF3

NCBI Official Synonym Symbols

GCSF; CSF3OS; C17orf33

UniProt Protein Name

Granulocyte colony-stimulating factor

UniProt Synonym Protein Names

Pluripoietin; INN: Filgrastim; INN: Lenograstim

Protein Family

Granulocyte colony-stimulating factor receptor

UniProt Gene Name

CSF3

UniProt Synonym Gene Names

C17orf33; GCSF; G-CSF

UniProt Entry Name

CSF3_HUMAN

NCBI Protein Information

granulocyte colony-stimulating factor

NCBI Summary for G-CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

UniProt Comments for G-CSF

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Research Articles on G-CSF

1. these data suggest that G-CSF may contribute to tumor growth and decrease the antitumor effect of radiotherapy, possibly by promoting vascularization in cancer lesions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with G-CSF active protein

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram

Diseases associated with G-CSF active protein

Disease Name	Pubmed Publications
Disease Susceptibility Antibodies	>7 publications with G-CSF and Disease Susceptibility
Nervous System Diseases Antibodies	>7 publications with G-CSF and Nervous System Diseases
Neutropenia Antibodies	>5 publications with G-CSF and Neutropenia
Leukopenia Antibodies	>3 publications with G-CSF and Leukopenia
Breast Neoplasms Antibodies	>3 publications with G-CSF and Breast Neoplasms
Leukemia, Myeloid, Acute Antibodies	>3 publications with G-CSF and Leukemia, Myeloid, Acute
Neoplasm Metastasis Antibodies	>3 publications with G-CSF and Neoplasm Metastasis
Brain Injuries Antibodies	>2 publications with G-CSF and Brain Injuries
Anemia Antibodies	>2 publications with G-CSF and Anemia
Hypertrophy Antibodies	>2 publications with G-CSF and Hypertrophy

Organs/Tissues associated with G-CSF active protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>9 publications with G-CSF and Muscle
Lung Antibodies	>9 publications with G-CSF and Lung
Skin Antibodies	>8 publications with G-CSF and Skin
Brain Antibodies	>4 publications with G-CSF and Brain
Uterus Antibodies	>3 publications with G-CSF and Uterus
Liver Antibodies	>2 publications with G-CSF and Liver
Intestine Antibodies	>1 publications with G-CSF and Intestine
Uncharacterized Tissue Antibodies	>1 publications with G-CSF and Uncharacterized Tissue