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FGF-21 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546405

Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose… More details

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Product Overview

Product Name
FGF-21, Active Protein
Full Product Name
Recombinant Rat Fibroblast Growth Factor-21 (rRtFGF-21)
Product Gene Name
FGF-21 active protein
Sequence
AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS
Host
Escherichia coli.
Form/Format
Lyophilized from a 0.2 um filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.
Purity/Purification
> 95% by SDS-PAGE and HPLC analyses.
Preparation and Storage
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Molecular Weight
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
OMIM
609436
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 ug/ml of rMuKlotho-β and 10 ug/ml of heparin.
Physical Appearance
White lyophilized powder.
Endotoxin
Less than 1EU/ug of rRtFGF-21 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of FGF-21 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FGF-21 purchase

MBS546405 is an active protein targeting FGF-21. Also known as Recombinant Rat Fibroblast Growth Factor-21 (rRtFGF-21). Host: Escherichia coli.

Additional Details

NCBI/Uniprot data below describe general gene information for FGF-21. It may not necessarily be applicable to this product.

NCBI GI #
9506597
NCBI GeneID
26291
NCBI Accession #
NP_061986.1
UniProt Secondary Accession #
Q8N683
UniProt Related Accession #
Q9NSA1
GenBank Nucleotide #
NM_019113.3
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Synonym Full Names
fibroblast growth factor 21
NCBI Official Symbol
FGF21
UniProt Protein Name
Fibroblast growth factor 21
Protein Family
Fibroblast growth factor
UniProt Gene Name
FGF21
UniProt Synonym Gene Names
FGF-21
UniProt Entry Name
FGF21_HUMAN
NCBI Protein Information
fibroblast growth factor 21

NCBI Summary for FGF-21

Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue. [provided by RefSeq, Mar 2016]
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UniProt Comments for FGF-21

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cell; extracellular region; extracellular space

Molecular Function: fibroblast growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell-cell signaling; positive regulation of cell proliferation; positive regulation of glucose import; positive regulation of low-density lipoprotein receptor biosynthetic process; response to activity; response to nutrient levels; signal transduction; unfolded protein response
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Research Articles on FGF-21

1. FGF21 mediates alcohol-induced adipose tissue lipolysis by activation of systemic release of catecholamin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FGF-21 active protein

 Reference Product  PubMed Publications
 KLB active protein  >69 publications with FGF-21 and KLB
 FGFR1 active protein  >53 publications with FGF-21 and FGFR1
 FGFR4 active protein  >24 publications with FGF-21 and FGFR4
 PPARA active protein  >8 publications with FGF-21 and PPARA
 CYP7A1 active protein  >7 publications with FGF-21 and CYP7A1
 FGFR3 active protein  >7 publications with FGF-21 and FGFR3
 LEP active protein  >2 publications with FGF-21 and LEP
 INSR active protein  >1 publications with FGF-21 and INSR

Diseases associated with FGF-21 active protein

 Disease Name  Pubmed Publications
 Obesity Antibodies  >308 publications with FGF-21 and Obesity
 Insulin Resistance Antibodies  >236 publications with FGF-21 and Insulin Resistance
 Fatty Liver Antibodies  >202 publications with FGF-21 and Fatty Liver
 Diabetes Mellitus Antibodies  >158 publications with FGF-21 and Diabetes Mellitus
 Liver Diseases Antibodies  >144 publications with FGF-21 and Liver Diseases
 Inflammation Antibodies  >88 publications with FGF-21 and Inflammation
 Cardiovascular Diseases Antibodies  >83 publications with FGF-21 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >69 publications with FGF-21 and Disease Models, Animal
 Weight Loss Antibodies  >60 publications with FGF-21 and Weight Loss
 Non-alcoholic Fatty Liver Disease Antibodies  >53 publications with FGF-21 and Non-alcoholic Fatty Liver Disease

Organs/Tissues associated with FGF-21 active protein

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >6 publications with FGF-21 and Lung
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