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CD16 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS550098

Human CD16 is encoded by two genes, FcγRIIIA and FcγRIIIB. FcγRIIIa is a transmembranous isoform of the receptor expressed on the surface of natural killer (NK) cells, macrophages, a subset of monocytes and of T cells in association with dimers of the… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
CD16, Monoclonal Antibody
Full Product Name
Monoclonal Mouse anti Human CD16
Product Gene Name
anti-CD16 antibody
Product Synonym Names
cd16; cd-16; monoclonal; mab; fcgr3aB
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
RKCD16
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Purity/Purification
The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
29,089 Da
OMIM
146740
3D Structure
ModBase 3D Structure for P08637
Host Note
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant human CD16a.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Product Categories/Family for anti-CD16 antibody

Monoclonal
Application Notes for anti-CD16 antibody
Western Blot: This monoclonal antibody has been tested in WB analysis of human CD16a,CD16b. A suitable range of concentrations of this antibody for WB detection is 0.5-2 ug/ml.

Other Notes

Small volumes of anti-CD16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD16 purchase

MBS550098 is an antibody targeting CD16. Also known as Monoclonal Mouse anti Human CD16. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD16. It may not necessarily be applicable to this product.

NCBI GI #
50726979
NCBI GeneID
2214
NCBI Accession #
NP_000560.5
UniProt Primary Accession #
P08637
UniProt Secondary Accession #
Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9
GenBank Nucleotide #
NM_000569.6
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-A isoform a
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt Synonym Protein Names
CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2
Protein Family
CD160 antigen
UniProt Gene Name
FCGR3A
UniProt Synonym Gene Names
CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
UniProt Entry Name
FCG3A_HUMAN

NCBI Summary for CD16

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD16

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: IgG binding

Biological Process: regulation of immune response; innate immune response; immune response

Disease: Immunodeficiency 20
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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