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Gm-CSF (VEGF) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS550101

GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for… More details

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Product Overview

Product Name
GM-CSF (VEGF), Monoclonal Antibody
Full Product Name
Monoclonal Mouse Anti Human GM-CSF
Product Gene Name
anti-VEGF antibody
Product Synonym Gene Name
GM-CSF
Product Synonym Names
Monoclonal Mouse Anti Human GMCSF; GM-CSF; gmcsf; csf2; Granulocyte Macrophage-Colony Stimulating Factor ; monoclonal
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
Purity/Purification
The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Preparation and Storage
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
16,295 Da
OMIM
138960
3D Structure
ModBase 3D Structure for P04141
Host Note
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human GM-CSF.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Product Categories/Family for anti-VEGF antibody

Monoclonal
Application Notes for anti-VEGF antibody
Neutralization: Data not available.
Western blot: This monoclonal antibody has been tested in WB analysis of human GM-CSF. A suitable range of concentrations of this antibody for WB detection is 0.5-2 ug/ml.
IHC: Use at a concentration of 1-2 ug/ml.

Other Notes

Small volumes of anti-VEGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VEGF purchase

MBS550101 is an antibody targeting VEGF. Also known as Monoclonal Mouse Anti Human GM-CSF. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VEGF. It may not necessarily be applicable to this product.

NCBI GI #
27437030
NCBI GeneID
1437
NCBI Accession #
NP_000749.2
UniProt Primary Accession #
P04141
UniProt Secondary Accession #
Q14CE8; Q2VPI8; Q8NFI6
GenBank Nucleotide #
NM_000758.3
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names
Colony-stimulating factor; CSF; Molgramostin; Sargramostim
Protein Family
Granulocyte colony-stimulating factor receptor
UniProt Gene Name
CSF2
UniProt Synonym Gene Names
GMCSF; GM-CSF; CSF
UniProt Entry Name
CSF2_HUMAN

NCBI Summary for VEGF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
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UniProt Comments for VEGF

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; immune response; myeloid dendritic cell differentiation; positive regulation of DNA replication
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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