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CD27 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS550104

CD27 is a disulfide-linked 120-kDa transmembrane glycoprotein expressed on the majority of T cells, B cells, and NK cells; it has homology to a family of molecules that includes the receptors for nerve growth factor and TNF. Previous studies strongly suggest… More details

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Technical Datasheet View PDF
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Product Overview

Product Name
CD27, Monoclonal Antibody
Full Product Name
Monoclonal Mouse anti Human CD27
Product Gene Name
anti-CD27 antibody
Product Synonym Names
mab; monoclonal; CD27; TNFRSF7 cd-27B
Clonality
Monoclonal
Isotype
IgG1
Clone Number
SBCD27
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
Purity/Purification
The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Preparation and Storage
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
29,137 Da
OMIM
186711
3D Structure
ModBase 3D Structure for P26842
Host Note
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human CD27.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Product Categories/Family for anti-CD27 antibody

Monoclonal
Application Notes for anti-CD27 antibody
Western Blot: This monoclonal antibody has been tested in WB analysis of human CD27. A suitable range of concentrations of this antibody for WB detection is 0.5-2 ug/ml.

Other Notes

Small volumes of anti-CD27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD27 purchase

MBS550104 is an antibody targeting CD27. Also known as Monoclonal Mouse anti Human CD27. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD27. It may not necessarily be applicable to this product.

NCBI GI #
4507587
NCBI GeneID
939
NCBI Accession #
NP_001233.1
UniProt Primary Accession #
P26842
UniProt Secondary Accession #
B2RDZ0
GenBank Nucleotide #
NM_001242.4
NCBI Official Full Name
CD27 antigen
UniProt Protein Name
CD27 antigen
UniProt Synonym Protein Names
CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7
Protein Family
CD27 antigen
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN

NCBI Summary for CD27

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD27

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity

Biological Process: release of cytoplasmic sequestered NF-kappaB; cell surface receptor linked signal transduction; response to ethanol; positive regulation of B cell differentiation; positive regulation of JNK cascade; immunoglobulin mediated immune response; negative regulation of caspase activity; negative regulation of apoptosis

Disease: Lymphoproliferative Syndrome 2
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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