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TPO Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS550367

MBS550367 is an antibody targeting TPO. Also known as TPO Antibody. Reactive in Human. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TPO, Polyclonal Antibody
Full Product Name
TPO Antibody
Product Gene Name
anti-TPO antibody
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Lyophilized from PBS, pH 7.2
3D Structure
ModBase 3D Structure for P07202
Immunogen
Recombinant Human TPO

Product Categories/Family for anti-TPO antibody

Antigen Affinity
Applications Tested/Suitable for anti-TPO antibody
ELISA (EIA), Western Blot (WB)

Sandwich ELISA of anti-TPO antibody

96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.
Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.
After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.
Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.

For Sandwich ELISA image: capture antibody ( MBS550367 ), biotinylated detection antibody ( MBS553608 )
Goat Anti-TPO Polyclonal Antibody – Cat# MBS550367

Western Blot (WB) of anti-TPO antibody

The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20, the membrane was incubated with primary antibody (at 4°C overnight.
Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies.
Enhanced chemiluminescence (ECL) reagents were used to visualize.

Recombinant immunogen ( MBS553717 ); primary antibody ( MBS550367 )
Goat Anti-TPO Polyclonal Antibody, Western Blot (WB) – Cat# MBS550367

Other Notes

Small volumes of anti-TPO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TPO. It may not necessarily be applicable to this product.

NCBI GI #
332078492
NCBI GeneID
7173
NCBI Accession #
NP_001193674.1
UniProt Primary Accession #
P07202
UniProt Related Accession #
P07202
GenBank Nucleotide #
NM_001206745.1
NCBI Official Full Name
thyroid peroxidase isoform b
NCBI Official Synonym Full Names
thyroid peroxidase
NCBI Official Symbol
TPO
NCBI Official Synonym Symbols
MSA; TPX; TDH2A
Protein Family
Thyroid peroxidase
NCBI Protein Information
thyroid peroxidase

NCBI Summary for TPO

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]
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Research Articles on TPO

1. Biochemical properties of the TPO protein expressed in cancer and normal breast tissue are similar to those observed for the thyroid TPO. Nevertheless, there are some differences in comparison with the thyroid-tissue expressed protein, such as a lower N-glycan content, a slightly smaller polypeptide length, a decreased enzymatic activity, and undetectable dimer formation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TPO antibody

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 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
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