• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Epidermal Growth Factor (EGF) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553002

Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha… More details

Please Inquire
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Epidermal Growth Factor (EGF), Recombinant Protein
Full Product Name
Mouse Epidermal Growth Factor Recombinant
Product Gene Name
EGF recombinant protein
Product Synonym Names
epidermal growth factor; egf
Sequence
STAPSLLGED GHHLDRNSYP GCPSSYDGYC LNGGVCMHIE SLDSYTCNCV IGYSGDRCQT RDLRWWELRH AGYGQKHDIM VVAVCMVALV LLLLLGMWGT YYYRTRKQLS NPPKNPCDEP
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Mouse EGF was lyophilized from a 0.2 um filtered solution in PBS, pH 7.5.
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
133,072 Da
3D Structure
ModBase 3D Structure for P01132
Domain
EGF
Host Note
Optimized DNA sequence encoding Mouse EGF mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Molecular Weight Note
Recombinant Mouse EGF, generated by the proteolytic removal of the signal peptide
and propeptide, is a disulfide-linked monomer protein.

Recombinant Mouse EGF consists of 54 amino acids and migrates as an approximately 6.2 kDa protein under reducing conditions.

Product Categories/Family for EGF recombinant protein

Cytokines

Other Notes

Small volumes of EGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EGF purchase

MBS553002 is a recombinant protein targeting EGF. Also known as Mouse Epidermal Growth Factor Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.

NCBI GI #
170172524
NCBI GeneID
13645
NCBI Accession #
NP_034243.2
UniProt Primary Accession #
P01132
UniProt Secondary Accession #
Q569W5; Q6P9J2; E9QNX6
UniProt Related Accession #
P01132; Q3UWD7; Q569W5; Q8VD07
GenBank Nucleotide #
NM_010113.3
NCBI Official Full Name
pro-epidermal growth factor
UniProt Protein Name
Pro-epidermal growth factor
Protein Family
Pro-epidermal growth factor
UniProt Gene Name
Egf
UniProt Synonym Gene Names
EGF
UniProt Entry Name
EGF_MOUSE

UniProt Comments for EGF

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase

Cellular Component: extracellular space; membrane; lysosomal membrane; plasma membrane; integral to membrane; intracellular

Molecular Function: protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; positive regulation of epidermal growth factor receptor activity; branching morphogenesis of a tube; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of granule cell precursor proliferation; STAT protein nuclear translocation; positive regulation of DNA binding
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with EGF recombinant protein

 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 EGFR Downregulation Pathway antibodies  EGFR Downregulation Pathway Diagram
 EGFR Interacts With Phospholipase C-gamma Pathway antibodies  EGFR Interacts With Phospholipase C-gamma Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram

Diseases associated with EGF recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7217 publications with EGF and Neoplasms
 Carcinoma Antibodies  >3632 publications with EGF and Carcinoma
 Breast Neoplasms Antibodies  >1440 publications with EGF and Breast Neoplasms
 Adenocarcinoma Antibodies  >1137 publications with EGF and Adenocarcinoma
 Gastrointestinal Diseases Antibodies  >1124 publications with EGF and Gastrointestinal Diseases
 Carcinoma, Squamous Cell Antibodies  >1071 publications with EGF and Carcinoma, Squamous Cell
 Necrosis Antibodies  >831 publications with EGF and Necrosis
 Neoplasms, Experimental Antibodies  >747 publications with EGF and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >640 publications with EGF and Nervous System Diseases
 Lung Neoplasms Antibodies  >640 publications with EGF and Lung Neoplasms

Organs/Tissues associated with EGF recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1635 publications with EGF and Liver
 Brain Antibodies  >1523 publications with EGF and Brain
 Kidney Antibodies  >1438 publications with EGF and Kidney
 Lung Antibodies  >1356 publications with EGF and Lung
 Skin Antibodies  >1184 publications with EGF and Skin
 Muscle Antibodies  >1159 publications with EGF and Muscle
 Embryonic Tissue Antibodies  >997 publications with EGF and Embryonic Tissue
 Intestine Antibodies  >805 publications with EGF and Intestine
 Ovary Antibodies  >718 publications with EGF and Ovary
 Prostate Antibodies  >612 publications with EGF and Prostate
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000