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Acidic Fibroblast Growth Factor (aFGF) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553019

aFGF and bFGF display 55% homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. Because aFGF binds to the same receptor as bFGF, aFGF displays more… More details

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Product Overview

Online Datasheet in PDF
Product Name
acidic Fibroblast Growth Factor (aFGF), Recombinant Protein
Full Product Name
Human acidic Fibroblast Growth Factor Recombinant
Product Gene Name
aFGF recombinant protein
Product Synonym Names
HBGF-1; afgf; Beta-endothelial cell growth factor
Sequence
MAEGEITTFT ALTEKFNLPP GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESVGEVY IKSTETGQYL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant acidic FGF was lyophilized from a 0.2 um filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.
Purity/Purification
>98%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
17,460 Da
OMIM
131220
3D Structure
ModBase 3D Structure for P05230
Domain
HBGF/IL1
Host Note
Optimized DNA sequence encoding Human acidic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human aFGF, generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated mass of 16 kDa.

Recombinant FGF acidic is a disulfide-linked monomer protein consisting of 141 amino acids and migrates as an approximately 16 kDa protein under reducing and non-reducing conditions.

Product Categories/Family for aFGF recombinant protein

Cytokines

Other Notes

Small volumes of aFGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for aFGF purchase

MBS553019 is a recombinant protein targeting aFGF. Also known as Human acidic Fibroblast Growth Factor Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for aFGF. It may not necessarily be applicable to this product.

NCBI GI #
4503697
NCBI GeneID
2246
NCBI Accession #
NP_000791.1
UniProt Primary Accession #
P05230
UniProt Secondary Accession #
P07502; Q16588; B2R5T0; D3DQF2
UniProt Related Accession #
P05230; Q16089; Q6LBM3; Q9UBK1; Q9UH63
GenBank Nucleotide #
NM_000800.4
NCBI Official Full Name
fibroblast growth factor 1 isoform 1
UniProt Protein Name
Fibroblast growth factor 1
UniProt Synonym Protein Names
Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1
Protein Family
Acidic fibroblast growth factor intracellular-binding protein
UniProt Gene Name
FGF1
UniProt Synonym Gene Names
FGFA; FGF-1; aFGF; ECGF; HBGF-1
UniProt Entry Name
FGF1_HUMAN

NCBI Summary for aFGF

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
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UniProt Comments for aFGF

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; extracellular space; proteinaceous extracellular matrix; extracellular region; nucleolus; cell cortex; cytosol

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with aFGF recombinant protein

 Reference Product  PubMed Publications
 FGFR1 recombinant protein  >195 publications with aFGF and FGFR1
 FGFR2 recombinant protein  >108 publications with aFGF and FGFR2
 FGFR3 recombinant protein  >54 publications with aFGF and FGFR3
 FGFR4 recombinant protein  >48 publications with aFGF and FGFR4
 S100A13 recombinant protein  >29 publications with aFGF and S100A13

Pathways associated with aFGF recombinant protein

 Products by Pathway  Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram
 FGFR1 Ligand Binding And Activation Pathway antibodies  FGFR1 Ligand Binding And Activation Pathway Diagram
 FGFR1b Ligand Binding And Activation Pathway antibodies  FGFR1b Ligand Binding And Activation Pathway Diagram
 FGFR1c Ligand Binding And Activation Pathway antibodies  FGFR1c Ligand Binding And Activation Pathway Diagram
 FGFR2 Ligand Binding And Activation Pathway antibodies  FGFR2 Ligand Binding And Activation Pathway Diagram
 FGFR2b Ligand Binding And Activation Pathway antibodies  FGFR2b Ligand Binding And Activation Pathway Diagram
 FGFR2c Ligand Binding And Activation Pathway antibodies  FGFR2c Ligand Binding And Activation Pathway Diagram
 FGFR3 Ligand Binding And Activation Pathway antibodies  FGFR3 Ligand Binding And Activation Pathway Diagram

Diseases associated with aFGF recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >330 publications with aFGF and Neoplasms
 Cardiovascular Diseases Antibodies  >116 publications with aFGF and Cardiovascular Diseases
 Neovascularization, Pathologic Antibodies  >85 publications with aFGF and Neovascularization, Pathologic
 Ischemia Antibodies  >83 publications with aFGF and Ischemia
 Breast Neoplasms Antibodies  >58 publications with aFGF and Breast Neoplasms
 Inflammation Antibodies  >43 publications with aFGF and Inflammation
 Adenocarcinoma Antibodies  >39 publications with aFGF and Adenocarcinoma
 Urologic Diseases Antibodies  >39 publications with aFGF and Urologic Diseases
 Genital Diseases, Male Antibodies  >33 publications with aFGF and Genital Diseases, Male
 Prostatic Diseases Antibodies  >32 publications with aFGF and Prostatic Diseases

Organs/Tissues associated with aFGF recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >266 publications with aFGF and Brain
 Muscle Antibodies  >227 publications with aFGF and Muscle
 Nerve Antibodies  >172 publications with aFGF and Nerve
 Embryonic Tissue Antibodies  >159 publications with aFGF and Embryonic Tissue
 Bone Antibodies  >116 publications with aFGF and Bone
 Liver Antibodies  >104 publications with aFGF and Liver
 Lung Antibodies  >104 publications with aFGF and Lung
 Heart Antibodies  >102 publications with aFGF and Heart
 Eye Antibodies  >102 publications with aFGF and Eye
 Kidney Antibodies  >77 publications with aFGF and Kidney
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