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Cytomegalovirus Pp65 (UL83) (CMV pp65) Cytomegalovirus Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553061

Human cytomegalovirus (HCMV) lower matrix protein pp65 (ppUL83), a major target for host anti-HCMV response, is the product of a late-expressed virus mRNA (reviewed in Spaete et al., 1994). pp65 replenishes a type of HCMV non-infectious particle, the dense… More details

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Product Overview

Product Name
Cytomegalovirus pp65 (UL83) (CMV pp65), Recombinant Protein
Full Product Name
Cytomegalovirus pp65 (UL83) Recombinant
Product Gene Name
CMV pp65 recombinant protein
Product Synonym Names
CMV; pp65; ul83
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Liquid
Purity/Purification
>95%, as determined by SDS-PAGE.
Preparation and Storage
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
62,898 Da
3D Structure
ModBase 3D Structure for P06725
Host Note
DNA sequence encoding immunodominant fragment of Cytomegalovirus 65kDa tegument phosphoprotein was expressed in Escherichia Coli.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant CMV pp65 (UL83) encodes the immunodominant sequences of the Cytomegalovirus matrix phosphoprotein. This molecule has a calculated mass of approximately 22kDa.

Recombinant CMV pp65 contains an N terminal GST purification tag and migrates as an approximately 52 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Product Categories/Family for CMV pp65 recombinant protein

Viral Antigens
Application Notes for CMV pp65 recombinant protein
CMV pp65 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.

Other Notes

Small volumes of CMV pp65 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CMV pp65 purchase

MBS553061 is a recombinant protein targeting CMV pp65. Also known as Cytomegalovirus pp65 (UL83) Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for CMV pp65. It may not necessarily be applicable to this product.

NCBI GI #
130714
NCBI Accession #
P06725.2
UniProt Primary Accession #
P06725
UniProt Secondary Accession #
Q7M6K8
NCBI Official Full Name
65 kDa phosphoprotein
UniProt Protein Name
65 kDa phosphoprotein
UniProt Synonym Protein Names
65 kDa matrix phosphoprotein; Phosphoprotein UL83; Tegument protein UL83
UniProt Gene Name
UL83
UniProt Synonym Gene Names
pp65
UniProt Entry Name
PP65_HCMVA

UniProt Comments for CMV pp65

Function: Counteracts the host antiviral immune response when activated and phosphorylated, by preventing IRF3 from entering the nucleus. Also participates in the transactivation of viral major immediate-early genes by the recruitment of host IFI16 to the promoters pf these genes. Ref.9 Ref.11

Subunit structure: Interacts with host NCL/nucleolin. Interacts with host IFI16. Ref.11 Ref.12

Subcellular location: Virion tegument

Potential. Host nucleus. Host cytoplasm. Note: As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection. The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection. Thereafter, pp65 is probably exported and accumulates in the cytoplasm. Also found in dense bodies. Ref.6 Ref.10

Post-translational modification: Phosphorylation may play a role in the localization of the protein.

Sequence similarities: Belongs to the herpesviridae pp65 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CMV pp65 recombinant protein

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