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PEDF Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553066

Pigment epithelium-derived factor (PEDF), a 50-kDa secreted glycoprotein, is widely expressed throughout the human body, with expression decreasing during human hepatocellular carcinoma and breast cancer progression.PEDF binds to a cell membrane receptor to… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PEDF, Recombinant Protein
Full Product Name
Human PEDF Recombinant
Product Gene Name
PEDF recombinant protein
Product Synonym Names
pedf; serpinf1
Sequence
FGYDLYRVRS STSPTTNVLL SPLSVATALS ALSLGAEQRT ESIIHRALYY DLISSPDIHG TYKELLDTVT APQKNLKSAS RIVFEKKLRI KSSFVAPLEK SYGTRPRVLT GNPRLDLQEI NNWVQAQMKG KLARSTKEIP DEISILLLGV AHFKGQWVTK FDSRKTSLED FYLDEERTVR VPMMSDPKAV LRYGLDSDLS CKIAQLPLTG SMSIIFFLPL KVTQNLTLIE ESLTSEFIHD IDRELKTVQA VLTVPKLKLS YEGEVTKSLQ EMKLQSLFDS PDFSKITGKP IKLTQVEHRA GFEWNEDGAG TTPSPGLQPA HLTFPLDYHL NQPFIFVLRD TDTGALLFIG KILDPRGP
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant PEDF was lyophilized from a 0.2 um filtered 20mM PB,150mM NaCl solution pH 7.5.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
46,312 Da
OMIM
172860
3D Structure
ModBase 3D Structure for P36955
Domain
SERPIN
Host Note
Optimized DNA sequence encoding Human PEDF mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human PEDF, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 45kDa.

Recombinant PEDF is a disulfide-linked monomer protein consisting of 440 amino acid residue subunits, and migrates as an approximately 45kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for PEDF recombinant protein

Cytokines

Other Notes

Small volumes of PEDF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEDF purchase

MBS553066 is a recombinant protein targeting PEDF. Also known as Human PEDF Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for PEDF. It may not necessarily be applicable to this product.

NCBI GI #
39725934
NCBI GeneID
5176
NCBI Accession #
NP_002606.3
UniProt Primary Accession #
P36955
UniProt Secondary Accession #
Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092
UniProt Related Accession #
P36955
GenBank Nucleotide #
NM_002615.5
NCBI Official Full Name
pigment epithelium-derived factor
UniProt Protein Name
Pigment epithelium-derived factor
UniProt Synonym Protein Names
Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1
Protein Family
Pigment epithelium-derived factor
UniProt Gene Name
SERPINF1
UniProt Synonym Gene Names
PEDF; PEDF
UniProt Entry Name
PEDF_HUMAN

NCBI Summary for PEDF

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]
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UniProt Comments for PEDF

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; melanosome; extracellular region; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; negative regulation of angiogenesis; positive regulation of neurogenesis; ovulation cycle; short-term memory; retina development in camera-type eye; negative regulation of inflammatory response; multicellular organismal development; response to arsenic; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with PEDF recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with PEDF and Neoplasms
 Carcinoma Antibodies  >2 publications with PEDF and Carcinoma
 Lung Diseases Antibodies  >2 publications with PEDF and Lung Diseases
 Leukemia Antibodies  >1 publications with PEDF and Leukemia
 Uterine Neoplasms Antibodies  >1 publications with PEDF and Uterine Neoplasms
 Proteinuria Antibodies  >1 publications with PEDF and Proteinuria
 Uterine Cervical Neoplasms Antibodies  >1 publications with PEDF and Uterine Cervical Neoplasms
 Lung Neoplasms Antibodies  >1 publications with PEDF and Lung Neoplasms
 Multiple Myeloma Antibodies  >1 publications with PEDF and Multiple Myeloma
 Adenocarcinoma Antibodies  >1 publications with PEDF and Adenocarcinoma

Organs/Tissues associated with PEDF recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >6 publications with PEDF and Nerve
 Eye Antibodies  >6 publications with PEDF and Eye
 Connective Tissue Antibodies  >3 publications with PEDF and Connective Tissue
 Bone Antibodies  >3 publications with PEDF and Bone
 Adipose Tissue Antibodies  >2 publications with PEDF and Adipose Tissue
 Lung Antibodies  >2 publications with PEDF and Lung
 Brain Antibodies  >2 publications with PEDF and Brain
 Kidney Antibodies  >2 publications with PEDF and Kidney
 Heart Antibodies  >1 publications with PEDF and Heart
 Embryonic Tissue Antibodies  >1 publications with PEDF and Embryonic Tissue
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