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Hepatitis C Virus Nonstructural Protein 3 (HCV NS3) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553077

The NS3 protein of hepatitis C virus (HCV) is a bifunctional protein containing a serine protease in the N-terminal one-third, which is stimulated upon binding of the NS4A cofactor, and an RNA helicase in the C-terminal. The C-terminal 450 amino acids of the… More details

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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Hepatitis C Virus Nonstructural protein 3 (HCV NS3), Recombinant Protein
Full Product Name
Hepatitis C Virus Nonstructural protein 3 Recombinant
Product Synonym Names
hcv; hepatitis; nonstructural; ns3
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
50mM Tris-HCl, pH-8, 60mM NaCl, 10mM glutathione, 0.25% sarkosil & 50% glycerol.
Purity/Purification
>95%, as determined by SDS-PAGE.
Preparation and Storage
This protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Domain
serine protease
Host Note
DNA sequence encoding immunodominant fragment of Hepatitis C Virus nonstructural protein 3 was expressed in Escherichia Coli.
Molecular Weight Note
Recombinant HCV NS3 encodes the immunodominant sequences of the HCV serine protease nonstructural protein. This molecule has a calculated mass of approximately 11kDa.

Recombinant HCV NS3 contains an N terminal GST purification tag and migrates as an approximately 37 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Product Categories/Family for HCV NS3 recombinant protein

Application Notes for HCV NS3 recombinant protein
HCV NS3 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Other Notes

Small volumes of HCV NS3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HCV NS3 purchase

MBS553077 is a recombinant protein targeting HCV NS3. Also known as Hepatitis C Virus Nonstructural protein 3 Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for HCV NS3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
nonstructural protein 3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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