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Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553079

GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF), Recombinant Protein
Full Product Name
Human Granulocyte Macrophage-Colony Stimulating Factor Recombinant
Product Gene Name
GM-CSF recombinant protein
Product Synonym Names
GM-CSF; gmcsf; csf2; Granulocyte Macrophage-Colony Stimulating Factor
Sequence
MWLQSLLLLG TVACSISAPA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA AEMNETVEVI SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH YKQHCPPTPE TSCATQIITF ESFKENLKDF LLVIPFDCWE PVQE
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Human GM-CSF was lyophilized from a 0.2 um filtered solution in PBS, pH 7.0.
Purity/Purification
>96%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
16,295 Da
OMIM
138960
3D Structure
ModBase 3D Structure for P04141
Domain
four helix cytokine core
Host Note
Optimized DNA sequence encoding Human Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human Granulocyte Macrophage-Colony Stimulating Factor is generated by the proteolytic removal of the signal peptide and propeptide,this molecule has a calculated mass of 15 kDa.

Recombinant GM-CSF is a disulfide-linked monomer protein, consisting of 128 amino acids and migrates as an approximately 15 kDa protein under reducing conditions.

Product Categories/Family for GM-CSF recombinant protein

Cytokines

Other Notes

Small volumes of GM-CSF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GM-CSF purchase

MBS553079 is a recombinant protein targeting GM-CSF. Also known as Human Granulocyte Macrophage-Colony Stimulating Factor Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for GM-CSF. It may not necessarily be applicable to this product.

NCBI GI #
27437030
NCBI GeneID
1437
NCBI Accession #
NP_000749.2
UniProt Primary Accession #
P04141
UniProt Secondary Accession #
Q14CE8; Q2VPI8; Q8NFI6
UniProt Related Accession #
P04141; Q647J8
GenBank Nucleotide #
NM_000758.3
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names
Colony-stimulating factor; CSF; Molgramostin; Sargramostim
Protein Family
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
CSF2
UniProt Synonym Gene Names
GMCSF; GM-CSF; CSF
UniProt Entry Name
CSF2_HUMAN

NCBI Summary for GM-CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
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UniProt Comments for GM-CSF

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; immune response; myeloid dendritic cell differentiation; positive regulation of DNA replication
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GM-CSF recombinant protein

 Reference Product  PubMed Publications
 CSF2RB recombinant protein  >6 publications with GM-CSF and CSF2RB
 CSF2RA recombinant protein  >4 publications with GM-CSF and CSF2RA
 KITLG recombinant protein  >2 publications with GM-CSF and KITLG
 IL3RA recombinant protein  >2 publications with GM-CSF and IL3RA
 SDC2 recombinant protein  >1 publications with GM-CSF and SDC2

Pathways associated with GM-CSF recombinant protein

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with GM-CSF recombinant protein

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >17 publications with GM-CSF and Necrosis
 Inflammation Antibodies  >14 publications with GM-CSF and Inflammation
 Neoplasms Antibodies  >10 publications with GM-CSF and Neoplasms
 Immune System Diseases Antibodies  >9 publications with GM-CSF and Immune System Diseases
 Respiratory Tract Diseases Antibodies  >6 publications with GM-CSF and Respiratory Tract Diseases
 Bacterial Infections Antibodies  >6 publications with GM-CSF and Bacterial Infections
 Leukemia Antibodies  >6 publications with GM-CSF and Leukemia
 Hematologic Diseases Antibodies  >6 publications with GM-CSF and Hematologic Diseases
 Lung Diseases Antibodies  >5 publications with GM-CSF and Lung Diseases
 Bone Marrow Diseases Antibodies  >5 publications with GM-CSF and Bone Marrow Diseases

Organs/Tissues associated with GM-CSF recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with GM-CSF and Blood
 Bone Antibodies  >6 publications with GM-CSF and Bone
 Lung Antibodies  >3 publications with GM-CSF and Lung
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