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Interferon-Gamma (IFN-gamma) Rat Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553083

IFN-gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFN gamma receptors are expressed on all types of human cells with… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Interferon-gamma (IFN-gamma), Recombinant Protein
Full Product Name
Rat Interferon-gamma Recombinant
Product Gene Name
IFN-gamma recombinant protein
Product Synonym Names
ifn-g; ifng; gamma interferon
Sequence
MSATRRVLVL QLCLMALSGC YCQGTLIESL ESLKNYFNSS SMDAMEGKSL LLDIWRNWQK DGNTKILESQ IISFYLRLFE VLKDNQAISN NISVIESHLI TNFFSNSKAK KDAFMSIAKF EVNNPQIQHK AVNELIRVIH QLSPESSLRK RKRSRC
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant Rat IFN-gamma was lyophilized from 0.2 um filtered PBS solution, pH 7.0.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
17,918 Da
3D Structure
ModBase 3D Structure for P01581
Host Note
Optimized DNA sequence encoding Human Rat Interferon gamma mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native Rat gamma Interferon is generated by the proteolytic removal of the signal peptide, the molecule has a calculated molecular mass of approximately 16 kDa. Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 135 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for IFN-gamma recombinant protein

Cytokines

Other Notes

Small volumes of IFN-gamma recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFN-gamma purchase

MBS553083 is a recombinant protein targeting IFN-gamma. Also known as Rat Interferon-gamma Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for IFN-gamma. It may not necessarily be applicable to this product.

NCBI GI #
20302055
NCBI GeneID
25712
NCBI Accession #
NP_620235.1
UniProt Primary Accession #
P01581
UniProt Related Accession #
P01581
GenBank Nucleotide #
NM_138880.2
NCBI Official Full Name
interferon gamma
UniProt Protein Name
Interferon gamma
Protein Family
Interferon gamma receptor
UniProt Gene Name
Ifng
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_RAT

NCBI Summary for IFN-gamma

an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006]

UniProt Comments for IFN-gamma

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Released primarily from activated T lymphocytes.

Sequence similarities: Belongs to the type II (or gamma) interferon family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with IFN-gamma recombinant protein

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with IFN-gamma recombinant protein

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >81 publications with IFN-gamma and Immune System Diseases
 Necrosis Antibodies  >68 publications with IFN-gamma and Necrosis
 Inflammation Antibodies  >60 publications with IFN-gamma and Inflammation
 Neoplasms Antibodies  >49 publications with IFN-gamma and Neoplasms
 Lung Diseases Antibodies  >30 publications with IFN-gamma and Lung Diseases
 Tuberculosis Antibodies  >28 publications with IFN-gamma and Tuberculosis
 Liver Diseases Antibodies  >19 publications with IFN-gamma and Liver Diseases
 Hepatitis Antibodies  >18 publications with IFN-gamma and Hepatitis
 Asthma Antibodies  >14 publications with IFN-gamma and Asthma
 Intestinal Diseases Antibodies  >10 publications with IFN-gamma and Intestinal Diseases
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