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Interleukin-1 Alpha (IL-1) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553101

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Interleukin-1 alpha (IL-1), Recombinant Protein
Full Product Name
Mouse Interleukin-1 alpha Recombinant
Product Gene Name
IL-1 recombinant protein
Product Synonym Names
il-1 alpha; il1a; il1; interleukin-1; il-1a
Sequence
MAKVPDLFED LKNCYSENED YSSAIDHLSL NQKSFYDASY GSLHETCTDQ FVSLRTSETS KMSNFTFKES RVTVSATSSN GKILKKRRLS FSETFTEDDL QSITHDLEET IQPRSAPYTY QSDLRYKLMK LVRQKFVMND SLNQTIYQDV DKHYLSTTWL NDLQQEVKFD MYAYSSGGDD SKYPVTLKIS DSQLFVSAQG EDQPVLLKEL PETPKLITGS ETDLIFFWKS INSKNYFTSA AYPELFIATK EQSRVHLARG LPSMTDFQIS
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant mouse Interleukin-1 alpha was lyophilized from a 0.2 um filtered PBS solution.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
31,023 Da
3D Structure
ModBase 3D Structure for P01582
Domain
IL1
Host Note
Optimized DNA sequence encoding Mouse Interleukin-1 alpha mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Mature Mouse IL-1 alpha, is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 18 kDa.

Recombinant IL-1a is a disulfide-linked monomer protein consisting of 157 amino acid residue subunits and migrates as an approximately 18 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Product Categories/Family for IL-1 recombinant protein

Cytokines

Other Notes

Small volumes of IL-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-1 purchase

MBS553101 is a recombinant protein targeting IL-1. Also known as Mouse Interleukin-1 alpha Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for IL-1. It may not necessarily be applicable to this product.

NCBI GI #
47059075
NCBI GeneID
16175
NCBI Accession #
NP_034684.2
UniProt Primary Accession #
P01582
UniProt Related Accession #
P01582; Q3U0Y6; Q62161
GenBank Nucleotide #
NM_010554.4
NCBI Official Full Name
interleukin-1 alpha
UniProt Protein Name
Interleukin-1 alpha
Protein Family
Interleukin
UniProt Gene Name
Il1a
UniProt Synonym Gene Names
IL-1 alpha
UniProt Entry Name
IL1A_MOUSE

UniProt Comments for IL-1

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Motility/polarity/chemotaxis; Cytokine

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; cytosol

Molecular Function: interleukin-1 receptor binding; copper ion binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; positive regulation of interleukin-6 production; fever; positive regulation of stress-activated MAPK cascade; negative regulation of cell proliferation; positive regulation of angiogenesis; connective tissue replacement during inflammatory response; response to copper ion; positive regulation of cell division; keratinization; positive regulation of prostaglandin secretion; positive regulation of transcription from RNA polymerase II promoter; immune response; regulation of sensory perception of pain; inflammatory response; positive regulation of cytokine secretion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with IL-1 recombinant protein

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with IL-1 recombinant protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >40 publications with IL-1 and Inflammation
 Necrosis Antibodies  >37 publications with IL-1 and Necrosis
 Immune System Diseases Antibodies  >35 publications with IL-1 and Immune System Diseases
 Neoplasms Antibodies  >32 publications with IL-1 and Neoplasms
 Skin Diseases Antibodies  >23 publications with IL-1 and Skin Diseases
 Lung Diseases Antibodies  >14 publications with IL-1 and Lung Diseases
 Cardiovascular Diseases Antibodies  >12 publications with IL-1 and Cardiovascular Diseases
 Hypersensitivity Antibodies  >11 publications with IL-1 and Hypersensitivity
 Osteoarthritis Antibodies  >10 publications with IL-1 and Osteoarthritis
 Carcinoma Antibodies  >9 publications with IL-1 and Carcinoma

Organs/Tissues associated with IL-1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >37 publications with IL-1 and Blood
 Skin Antibodies  >13 publications with IL-1 and Skin
 Bone Marrow Antibodies  >2 publications with IL-1 and Bone Marrow
 Spleen Antibodies  >1 publications with IL-1 and Spleen
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