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Cd16b (Fcgr3b) (CD16) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553103

Human CD16 is encoded by two genes, FcγRIIIA and FcγRIIIB. FcγRIIIa is a transmembranous isoform of the receptor expressed on the surface of natural killer (NK) cells, macrophages, a subset of monocytes and of T cells in association with dimers of the… More details

$335 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD16b (FcgR3b), Recombinant Protein
Full Product Name
Human CD16b Recombinant (FcgR3b)
Product Gene Name
CD16 recombinant protein
Product Synonym Names
Human CD16b Recombinant (FcgrR3b) ; cd16; cd-16
Host
HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant CD16b is supplied as a lyophilized 0.2 um filtered PBS solution, pH7.4 containing 5% trehalose.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
Recombinant CD16b, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree to -70 degree C for twelve months.

Avoid repeated freeze/thaw cycles.
Molecular Weight
26,216 Da
OMIM
610665
3D Structure
ModBase 3D Structure for O75015
Domain
Ig-like C2
Host Note
Optimized DNA sequence encoding Human extracellular domain of human CD16b including a C-terminal 6His tag was expressed in HEK293 cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Molecular Weight Note
Recombinant CD16b is a monomer protein consisting of 195 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.

Product Categories/Family for CD16 recombinant protein

Receptors CD

Other Notes

Small volumes of CD16 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD16 purchase

MBS553103 is a recombinant protein targeting CD16. Also known as Human CD16b Recombinant (FcgR3b). Host: HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for CD16. It may not necessarily be applicable to this product.

NCBI GI #
132814489
NCBI GeneID
2215
NCBI Accession #
NP_000561.3
UniProt Primary Accession #
O75015
UniProt Related Accession #
O75015; Q9ULV2; Q9UPY7
GenBank Nucleotide #
NM_000570.4
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
UniProt Synonym Protein Names
Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
UniProt Entry Name
FCG3B_HUMAN

NCBI Summary for CD16

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]
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UniProt Comments for CD16

Function: Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Subunit structure: Monomer. Interacts with INPP5D/SHIP1

By similarity.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor. Secreted. Note: Secreted after cleavage.

Tissue specificity: Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils.

Post-translational modification: Glycosylated. Glycosylation plays an inhibitory role in the interaction with IgG3.The soluble form is produced by a proteolytic cleavage.

Polymorphism: There are three allelic forms of FCGR3B: FCGR3B*01 (NA-1), FCGR3B*02 (HNA-1b, NA-2) (shown here) and SH. FCGR3B*01 and FCGR3B*02 are detectable with antibodies against the biallelic neutrophil-specific antigen system NA. The more active FCGR3B*01 allele has been associated with severe renal disease in certain systemic vasculitides.

Miscellaneous: Encoded by one of two nearly indentical genes: FCGR3A and FCGR3B (Shown here) which are expressed in a tissue-specific manner. The 'Phe-203' in FCGR3A determines the transmembrane domains whereas the Ser-203 in FCGR3B determines the GPI-anchoring.

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD16 recombinant protein

 Reference Product  PubMed Publications
 APCS recombinant protein  >3 publications with CD16 and APCS

Pathways associated with CD16 recombinant protein

 Products by Pathway  Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram

Diseases associated with CD16 recombinant protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >33 publications with CD16 and Inflammation
 Cardiovascular Diseases Antibodies  >29 publications with CD16 and Cardiovascular Diseases
 Lupus Nephritis Antibodies  >12 publications with CD16 and Lupus Nephritis
 Sepsis Antibodies  >7 publications with CD16 and Sepsis
 Anemia Antibodies  >6 publications with CD16 and Anemia
 Wegener Granulomatosis Antibodies  >6 publications with CD16 and Wegener Granulomatosis
 Agranulocytosis Antibodies  >5 publications with CD16 and Agranulocytosis
 Mental Disorders Antibodies  >2 publications with CD16 and Mental Disorders
 Kidney Failure, Chronic Antibodies  >2 publications with CD16 and Kidney Failure, Chronic
 Hepatitis Antibodies  >2 publications with CD16 and Hepatitis

Organs/Tissues associated with CD16 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >182 publications with CD16 and Blood
 Kidney Antibodies  >14 publications with CD16 and Kidney
 Bone Marrow Antibodies  >10 publications with CD16 and Bone Marrow
 Lung Antibodies  >9 publications with CD16 and Lung
 Heart Antibodies  >5 publications with CD16 and Heart
 Placenta Antibodies  >3 publications with CD16 and Placenta
 Spleen Antibodies  >1 publications with CD16 and Spleen
 Brain Antibodies  >1 publications with CD16 and Brain
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