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Interleukin-18 (IL-18) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553113

When you are researching inflammation, you need highly purified recombinant proteins as neutralizing or positive controls and to generate DIY antibody reagents. MyBiosource has the capability to provide recombinant human interleukin 18 (human IL 18) from cell… More details

$240 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin-18 (IL-18), Active Protein
Full Product Name
Human Interleukin-18 Recombinant
Product Gene Name
IL-18 active protein
Product Synonym Names
IGIF; IL1F4; IL18; il-18
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant Interleukin-18 was lyophilized from 0.2 um filtered PBS solution pH 7.4.
Purity/Purification
>92%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 1 year from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°-8°C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
OMIM
600953
3D Structure
ModBase 3D Structure for Q14116
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source Note
Optimized DNA sequence encoding Human Interleukin-18 mature chain was expressed in Escherichia Coli
Presentation
Recombinant Interleukin-18 was lyophilized from 0.2 um filtered PBS solution pH 7.4.
Biological Activity
The ED(50) was determined by the dose-dependent proliferation ofhuman PBMC co-stimulated with IL-12 cells was found to be in the range of 5ng/ml.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Molecular Weight Note
Native human Interleukin-18, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 18 kDa. Recombinant Interleukin-18 is a disulfide-linked monomer protein consisting of 158 amino acid residue subunits, migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for IL-18 active protein

Cytokines

Other Notes

Small volumes of IL-18 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-18. It may not necessarily be applicable to this product.

NCBI GI #
342349321
NCBI GeneID
3606
NCBI Accession #
NP_001230140.1
UniProt Primary Accession #
Q14116
UniProt Secondary Accession #
O75599; Q6FGY3; Q6WWJ7
UniProt Related Accession #
Q14116; Q6WWJ7; Q96KJ8; Q9NQ49
GenBank Nucleotide #
NM_001243211.1
NCBI Official Full Name
interleukin-18 isoform 2 proprotein
UniProt Protein Name
Interleukin-18
UniProt Synonym Protein Names
Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma
Protein Family
Interleukin-18-binding protein
UniProt Gene Name
IL18
UniProt Synonym Gene Names
IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
UniProt Entry Name
IL18_HUMAN

NCBI Summary for IL-18

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
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UniProt Comments for IL-18

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; positive regulation of interleukin-17 production; regulation of cell adhesion; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL-18 active protein

 Reference Product  PubMed Publications
 CASP1 active protein  >342 publications with IL-18 and CASP1
 IL18R1 active protein  >198 publications with IL-18 and IL18R1
 CASP3 active protein  >48 publications with IL-18 and CASP3
 IL18RAP active protein  >24 publications with IL-18 and IL18RAP
 IL18BP active protein  >13 publications with IL-18 and IL18BP
 IL1RL2 active protein  >4 publications with IL-18 and IL1RL2
 CASP4 active protein  >4 publications with IL-18 and CASP4

Pathways associated with IL-18 active protein

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
 IL12 Signaling Mediated By STAT4 Pathway antibodies  IL12 Signaling Mediated By STAT4 Pathway Diagram

Diseases associated with IL-18 active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >824 publications with IL-18 and Inflammation
 Cardiovascular Diseases Antibodies  >319 publications with IL-18 and Cardiovascular Diseases
 Arthritis, Rheumatoid Antibodies  >190 publications with IL-18 and Arthritis, Rheumatoid
 Kidney Diseases Antibodies  >170 publications with IL-18 and Kidney Diseases
 Intestinal Diseases Antibodies  >160 publications with IL-18 and Intestinal Diseases
 Carcinoma Antibodies  >136 publications with IL-18 and Carcinoma
 Diabetes Mellitus Antibodies  >125 publications with IL-18 and Diabetes Mellitus
 Atherosclerosis Antibodies  >119 publications with IL-18 and Atherosclerosis
 Myocardial Ischemia Antibodies  >106 publications with IL-18 and Myocardial Ischemia
 Asthma Antibodies  >97 publications with IL-18 and Asthma

Organs/Tissues associated with IL-18 active protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >293 publications with IL-18 and Liver
 Lung Antibodies  >238 publications with IL-18 and Lung
 Skin Antibodies  >162 publications with IL-18 and Skin
 Bone Antibodies  >159 publications with IL-18 and Bone
 Brain Antibodies  >150 publications with IL-18 and Brain
 Heart Antibodies  >139 publications with IL-18 and Heart
 Intestine Antibodies  >122 publications with IL-18 and Intestine
 Bone Marrow Antibodies  >103 publications with IL-18 and Bone Marrow
 Muscle Antibodies  >99 publications with IL-18 and Muscle
 Connective Tissue Antibodies  >44 publications with IL-18 and Connective Tissue
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