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Toxoplasma Gondii Rhoptry Protein (TOXGO ROP4) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553140

ROP4, a previously uncharacterized member of the ROP2 family of proteins. ROP4 is secreted from the rhoptries during or shortly after invasion and associates with the PVM, presumably through its predicted transmembrane domain. ROP4 becomes phosphorylated in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Toxoplasma Gondii Rhoptry protein (TOXGO ROP4), Recombinant Protein
Full Product Name
Toxoplasma Gondii Rhoptry protein Recombinant
Product Gene Name
TOXGO ROP4 recombinant protein
Product Synonym Names
toxoplasma gondii; rop4; Rhoptry; toxgo
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.
Purity/Purification
>95%, as determined by SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
64,003 Da
3D Structure
ModBase 3D Structure for Q9U0D6
Host Note
DNA sequence encoding immunodominant fragment of Toxoplasma ROP4 protein was expressed in Escherichia Coli.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant TOXGO ROP4 encodes the immunodominant sequences of the Toxoplasma Dense Granule protein.

Recombinant ROP4 contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Product Categories/Family for TOXGO ROP4 recombinant protein

Viral Antigens
Application Notes for TOXGO ROP4 recombinant protein
Toxoplasma ROP4 antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma with minimal specificity problems.

Other Notes

Small volumes of TOXGO ROP4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TOXGO ROP4 purchase

MBS553140 is a recombinant protein targeting TOXGO ROP4. Also known as Toxoplasma Gondii Rhoptry protein Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for TOXGO ROP4. It may not necessarily be applicable to this product.

NCBI GI #
75025183
NCBI Accession #
Q9U0D6
UniProt Primary Accession #
Q9U0D6
UniProt Secondary Accession #
Q9U0D6
UniProt Protein Name
Rhoptry protein 4
UniProt Gene Name
ROP4
UniProt Entry Name
ROP4_TOXGO

UniProt Comments for TOXGO ROP4

Function: Thought to play a role in parasitophorous vacuole membrane function during the infection of host organisms. Ref.1

Subcellular location: Secreted. Parasitophorous vacuole membrane. Note: Localized to rhoptries which are secretory organelles at the apical end of the parasite and secreted during host cell invasion when it becomes associated with the parasitophorous vacuole membrane. Ref.1

Post-translational modification: Phosphorylated on multiple serine and threonine residues in parasitic extracts and infected cells but not in extracellular parasites. Ref.1

Sequence similarities: Contains 1 protein kinase domain.

Caution: Lacks the active site residue so is predicted to be catalytically inactive. Ref.1
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TOXGO ROP4 recombinant protein

 Reference Product  PubMed Publications
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