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MIP-1 Alpha (CCL3) (MIP-1 alpha) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553148

Macrophage Inflammatory Protein-1 is a factor produced by macrophages that causes local inflammatory responses, and induces superoxide production by neutrophils. Two peptides are responsible for this activity. They have been termed MIP-1-alpha, and MIP-… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MIP-1 alpha (CCL3), Recombinant Protein
Full Product Name
Human MIP-1 alpha (CCL3) Recombinant
Product Gene Name
MIP-1 alpha recombinant protein
Product Synonym Gene Name
MIP-1
Product Synonym Names
Human Macrophage Inflammatory protein-1 alpha Recombinant; ccl3; mip-1; MIP1A; SCYA3; Macrophage Inflammatory protein
Sequence
MQVSTAALAV LLCTMALCNQ FSASLAADTP TACCFSYTSR QIPQNFIADY FETSSQCSKP GVIFLTKRSR QVCADPSEEW VQKYVSDLEL SA
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant MIP-1 was lyophilized from a 0.2 um filtered PBS solution pH 7.5.
Purity/Purification
>98%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
10,085 Da
OMIM
182283
3D Structure
ModBase 3D Structure for P10147
Domain
IL8
Host Note
Optimized DNA sequence encoding Human MIP-1 alpha mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human MIP-1 alpha is generated by the proteolytic removal of the signal peptide. This molecule has a calculated molecular mass of approximately 8 kDa.

Recombinant MIP-1 alpha is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for MIP-1 alpha recombinant protein

Cytokines

Other Notes

Small volumes of MIP-1 alpha recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIP-1 alpha purchase

MBS553148 is a recombinant protein targeting MIP-1 alpha. Also known as Human MIP-1 alpha (CCL3) Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for MIP-1 alpha. It may not necessarily be applicable to this product.

NCBI GI #
4506843
NCBI GeneID
6348
NCBI Accession #
NP_002974.1
UniProt Primary Accession #
P10147
UniProt Related Accession #
P10147; Q14745
GenBank Nucleotide #
NM_002983.2
NCBI Official Full Name
C-C motif chemokine 3
UniProt Protein Name
C-C motif chemokine 3
UniProt Synonym Protein Names
G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinCleaved into the following chain:MIP-1-alpha(4-69); Alternative name(s):; LD78-alpha(4-69)
UniProt Gene Name
CCL3
UniProt Synonym Gene Names
G0S19-1; MIP1A; SCYA3; MIP-1-alpha
UniProt Entry Name
CCL3_HUMAN

NCBI Summary for MIP-1 alpha

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]
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UniProt Comments for MIP-1 alpha

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; CCR5 chemokine receptor binding; chemoattractant activity; protein kinase activity; phospholipase activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; response to toxin; behavior; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; monocyte chemotaxis; negative regulation of bone mineralization; regulation of cell shape; positive chemotaxis; cell-cell signaling; positive regulation of neuron apoptosis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; MAPKKK cascade; calcium-mediated signaling; cytoskeleton organization and biogenesis; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; positive regulation of tumor necrosis factor production; macrophage chemotaxis; osteoblast differentiation; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; cell activation; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MIP-1 alpha recombinant protein

 Reference Product  PubMed Publications
 CCR5 recombinant protein  >418 publications with MIP-1 alpha and CCR5
 CCR1 recombinant protein  >232 publications with MIP-1 alpha and CCR1
 CCR3 recombinant protein  >60 publications with MIP-1 alpha and CCR3
 CCR4 recombinant protein  >36 publications with MIP-1 alpha and CCR4
 TGFB1 recombinant protein  >28 publications with MIP-1 alpha and TGFB1
 BCL6 recombinant protein  >3 publications with MIP-1 alpha and BCL6
 CEBPB recombinant protein  >1 publications with MIP-1 alpha and CEBPB

Pathways associated with MIP-1 alpha recombinant protein

 Products by Pathway  Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram

Diseases associated with MIP-1 alpha recombinant protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >618 publications with MIP-1 alpha and Inflammation
 Nervous System Diseases Antibodies  >194 publications with MIP-1 alpha and Nervous System Diseases
 Lung Diseases Antibodies  >181 publications with MIP-1 alpha and Lung Diseases
 HIV Infections Antibodies  >161 publications with MIP-1 alpha and HIV Infections
 Cardiovascular Diseases Antibodies  >123 publications with MIP-1 alpha and Cardiovascular Diseases
 Vascular Diseases Antibodies  >114 publications with MIP-1 alpha and Vascular Diseases
 Skin Diseases Antibodies  >75 publications with MIP-1 alpha and Skin Diseases
 Leukemia Antibodies  >73 publications with MIP-1 alpha and Leukemia
 Arthritis, Rheumatoid Antibodies  >69 publications with MIP-1 alpha and Arthritis, Rheumatoid
 Liver Diseases Antibodies  >65 publications with MIP-1 alpha and Liver Diseases

Organs/Tissues associated with MIP-1 alpha recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1186 publications with MIP-1 alpha and Blood
 Lung Antibodies  >245 publications with MIP-1 alpha and Lung
 Brain Antibodies  >179 publications with MIP-1 alpha and Brain
 Liver Antibodies  >159 publications with MIP-1 alpha and Liver
 Kidney Antibodies  >60 publications with MIP-1 alpha and Kidney
 Heart Antibodies  >56 publications with MIP-1 alpha and Heart
 Eye Antibodies  >39 publications with MIP-1 alpha and Eye
 Intestine Antibodies  >39 publications with MIP-1 alpha and Intestine
 Uterus Antibodies  >17 publications with MIP-1 alpha and Uterus
 Placenta Antibodies  >14 publications with MIP-1 alpha and Placenta
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