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Cd32b (Fcgr2b) (CD32) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553152

Human CD32B (FcγRIIB), the low-affinity inhibitory Fcγ receptor (FcγR), is highly homologous in its extracellular domain to CD32A (FcγRIIA), an activating FcγR.CD32B is coexpressed with activating Fc receptors on monocytes, macrophages, basophils and… More details

$335 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD32b (FcgR2b), Active Protein
Full Product Name
Human CD32b Recombinant (FcgR2b)
Product Gene Name
CD32 active protein
Product Synonym Names
Human CD32b Recombinant (Fcg RIIb) ; Human CD32b Recombinant (Fcgamma RIIb) ; cd32; cd-32; fcgr3a
Host
HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant CD32b is supplied as a lyophilized 0.2 um filtered PBS solution, pH7.2.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
Recombinant CD32b, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.

Avoid repeated freeze/thaw cycles.
OMIM
152700
3D Structure
ModBase 3D Structure for P31994
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Host Note
Optimized DNA sequence encoding Human extracellular domain of human CD32b including a C-terminal 6His tag was expressed in HEK293 cells.
Biological Activity
The activity was determined by the ability to bind human IgG2 with a linear range of 0.1-6 ug/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Molecular Weight Note
Recombinant CD32b is a monomer protein consisting of 183 amino acid residue subunits, due to glycosylation migrates as an approximately 30 kDa protein on SDS-PAGE.

Product Categories/Family for CD32 active protein

Receptors CD

Other Notes

Small volumes of CD32 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD32 purchase

MBS553152 is an active protein targeting CD32. Also known as Human CD32b Recombinant (FcgR2b). Host: HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for CD32. It may not necessarily be applicable to this product.

NCBI GI #
50511932
NCBI GeneID
2213
NCBI Accession #
NP_001002273.1
UniProt Primary Accession #
P31994
UniProt Secondary Accession #
O95649; Q53X85; Q5VXA9; Q8NIA1; A6H8N3
UniProt Related Accession #
P31994; Q8IU78; Q8IU79; Q8IXE8; Q8IXE9; Q8NIA0
GenBank Nucleotide #
NM_001002273.2
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-b isoform 2
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-b
UniProt Synonym Protein Names
CDw32; Fc-gamma RII-b
UniProt Gene Name
FCGR2B
UniProt Synonym Gene Names
CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b
UniProt Entry Name
FCG2B_HUMAN

NCBI Summary for CD32

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
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UniProt Comments for CD32

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.

Protein type: Cell surface; Membrane protein, integral; Oncoprotein

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; IgG binding

Biological Process: regulation of immune response; viral reproduction; immune response; signal transduction

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD32 active protein

 Reference Product  PubMed Publications
 APCS active protein  >4 publications with CD32 and APCS
 CRP active protein  >3 publications with CD32 and CRP
 MAPK1 active protein  >1 publications with CD32 and MAPK1
 LYN active protein  >1 publications with CD32 and LYN
 BLK active protein  >1 publications with CD32 and BLK

Pathways associated with CD32 active protein

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram

Diseases associated with CD32 active protein

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >88 publications with CD32 and Autoimmune Diseases
 Lupus Erythematosus, Systemic Antibodies  >48 publications with CD32 and Lupus Erythematosus, Systemic
 Inflammation Antibodies  >34 publications with CD32 and Inflammation
 Kidney Diseases Antibodies  >17 publications with CD32 and Kidney Diseases
 Neoplasms Antibodies  >14 publications with CD32 and Neoplasms
 Lung Diseases Antibodies  >11 publications with CD32 and Lung Diseases
 Necrosis Antibodies  >11 publications with CD32 and Necrosis
 Blood Coagulation Disorders Antibodies  >9 publications with CD32 and Blood Coagulation Disorders
 Leukemia Antibodies  >6 publications with CD32 and Leukemia
 Asthma Antibodies  >5 publications with CD32 and Asthma

Organs/Tissues associated with CD32 active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >119 publications with CD32 and Blood
 Spleen Antibodies  >20 publications with CD32 and Spleen
 Bone Marrow Antibodies  >14 publications with CD32 and Bone Marrow
 Lymph Node Antibodies  >12 publications with CD32 and Lymph Node
 Kidney Antibodies  >9 publications with CD32 and Kidney
 Lung Antibodies  >7 publications with CD32 and Lung
 Brain Antibodies  >6 publications with CD32 and Brain
 Liver Antibodies  >4 publications with CD32 and Liver
 Connective Tissue Antibodies  >2 publications with CD32 and Connective Tissue
 Skin Antibodies  >2 publications with CD32 and Skin
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