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MIP-3 Alpha (CCL20) (MIP-3A) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553195

Macrophage inflammatory protein (MIP) -3/CCL20, also known as liver and activation-regulated chemokine (LARC) or Exodus, is a member of the CC chemokine subfamily initially noted to be expressed in human liver, lung, appendix, and tonsillar crypts. MIP-3 is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MIP-3 alpha (CCL20) (MIP-3A), Recombinant Protein
Full Product Name
Human MIP-3 alpha (CCL20) Recombinant
Product Gene Name
MIP-3A recombinant protein
Product Synonym Gene Name
MIP3
Product Synonym Names
Human MIP-3A (CCL20) Recombinant; ccl-20 ; CCL20; mip3a; mip-3a; SCYA20 ; LARC
Sequence
MCCTKSLLLA ALMSVLLLHL CGESEAASNF DCCLGYTDRI LHPKFIVGFT RQLANEGCDI NAIIFHTKKK LSVCANPKQT WVKYIVRLLS KKVKNM
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant CCL20 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
Purity/Purification
>98%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
10,762 Da
OMIM
601960
3D Structure
ModBase 3D Structure for P78556
Domain
IL8
Host Note
Optimized DNA sequence encoding Human MIP-3 alpha (CCL20) mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Human CCL20, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 8 kDa.

Recombinant CCL-20 is a disulfide-linked monomeric protein consisting of 71 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Product Categories/Family for MIP-3A recombinant protein

Cytokines

Other Notes

Small volumes of MIP-3A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIP-3A purchase

MBS553195 is a recombinant protein targeting MIP-3A. Also known as Human MIP-3 alpha (CCL20) Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for MIP-3A. It may not necessarily be applicable to this product.

NCBI GI #
194239688
NCBI GeneID
6364
NCBI Accession #
NP_001123518.1
UniProt Primary Accession #
P78556
UniProt Secondary Accession #
Q53S51; Q99664
UniProt Related Accession #
P78556
GenBank Nucleotide #
NM_001130046.1
NCBI Official Full Name
C-C motif chemokine 20 isoform 2
UniProt Protein Name
C-C motif chemokine 20
UniProt Synonym Protein Names
Beta-chemokine exodus-1; CC chemokine LARC; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20
UniProt Gene Name
CCL20
UniProt Synonym Gene Names
LARC; MIP3A; SCYA20; MIP-3-alpha
UniProt Entry Name
CCL20_HUMAN

NCBI Summary for MIP-3A

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
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UniProt Comments for MIP-3A

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli 25922 and S.aureus 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; wound healing; defense response to bacterium; immune response; inflammatory response; chemotaxis; positive regulation of interleukin-1 alpha biosynthetic process; signal transduction; positive regulation of nitric-oxide synthase biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MIP-3A recombinant protein

 Reference Product  PubMed Publications
 CCR6 recombinant protein  >267 publications with MIP-3A and CCR6

Pathways associated with MIP-3A recombinant protein

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram

Diseases associated with MIP-3A recombinant protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >199 publications with MIP-3A and Inflammation
 Neoplasms Antibodies  >94 publications with MIP-3A and Neoplasms
 Skin Diseases Antibodies  >61 publications with MIP-3A and Skin Diseases
 Intestinal Diseases Antibodies  >55 publications with MIP-3A and Intestinal Diseases
 Lung Diseases Antibodies  >44 publications with MIP-3A and Lung Diseases
 Arthritis, Rheumatoid Antibodies  >35 publications with MIP-3A and Arthritis, Rheumatoid
 Nervous System Diseases Antibodies  >34 publications with MIP-3A and Nervous System Diseases
 Asthma Antibodies  >23 publications with MIP-3A and Asthma
 Colitis Antibodies  >21 publications with MIP-3A and Colitis
 Demyelinating Diseases Antibodies  >19 publications with MIP-3A and Demyelinating Diseases

Organs/Tissues associated with MIP-3A recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >338 publications with MIP-3A and Blood
 Intestine Antibodies  >81 publications with MIP-3A and Intestine
 Lung Antibodies  >66 publications with MIP-3A and Lung
 Vascular Antibodies  >46 publications with MIP-3A and Vascular
 Liver Antibodies  >44 publications with MIP-3A and Liver
 Mouth Antibodies  >22 publications with MIP-3A and Mouth
 Brain Antibodies  >19 publications with MIP-3A and Brain
 Kidney Antibodies  >19 publications with MIP-3A and Kidney
 Uterus Antibodies  >15 publications with MIP-3A and Uterus
 Eye Antibodies  >13 publications with MIP-3A and Eye
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