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Interleukin-33 (IL-33) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553201

IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-B and all three MAPKs: p38, ERK1/2, and JNK1/2 in mast cells.IL-33 is expressed in multiple… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin-33 (IL-33), Recombinant Protein
Full Product Name
Human Interleukin-33 Recombinant
Product Gene Name
IL-33 recombinant protein
Product Synonym Gene Name
IL33
Product Synonym Names
IL1F11; NFHEV; interleukin-33; il33; il-33
Sequence
RRETTKRPSL KTGRKHKRHL VLAACQQQST VECFAFGISG VQKYTRALHD SSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant Interleukin-33 was lyophilized from 0.2 um filtered 1mg/ml in PBS buffer.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
30,759 Da
OMIM
608678
3D Structure
ModBase 3D Structure for O95760
Domain
Actin Binding
Host Note
Optimized DNA sequence encoding Human Interleukin-33 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human Interleukin-33, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18kDa.

Recombinant Interleukin-33 is a disulfide-linked monomer protein consisting of 160 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for IL-33 recombinant protein

Cytokines

Other Notes

Small volumes of IL-33 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-33 purchase

MBS553201 is a recombinant protein targeting IL-33. Also known as Human Interleukin-33 Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for IL-33. It may not necessarily be applicable to this product.

NCBI GI #
313851030
NCBI GeneID
90865
NCBI Accession #
NP_001186569.1
UniProt Primary Accession #
O95760
UniProt Secondary Accession #
Q2YEJ5; B4DJ35; B4E1Q9; D3DRI5; E7EAX4
UniProt Related Accession #
P60983; Q59G69; Q9BS35
GenBank Nucleotide #
NM_001199640.1
NCBI Official Full Name
interleukin-33 isoform 2
UniProt Protein Name
Interleukin-33
UniProt Synonym Protein Names
Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules
Protein Family
Interleukin
UniProt Gene Name
IL33
UniProt Synonym Gene Names
C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV
UniProt Entry Name
IL33_HUMAN

NCBI Summary for IL-33

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
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UniProt Comments for IL-33

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: protein binding; cytokine activity

Biological Process: negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; positive regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; negative regulation of interferon-gamma production; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with IL-33 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with IL-33 and Neoplasms
 Necrosis Antibodies  >3 publications with IL-33 and Necrosis
 Gonadal Disorders Antibodies  >2 publications with IL-33 and Gonadal Disorders
 Cataract Antibodies  >1 publications with IL-33 and Cataract
 Bone Diseases, Developmental Antibodies  >1 publications with IL-33 and Bone Diseases, Developmental
 Carcinoma Antibodies  >1 publications with IL-33 and Carcinoma
 Eye Abnormalities Antibodies  >1 publications with IL-33 and Eye Abnormalities
 Congenital Abnormalities Antibodies  >1 publications with IL-33 and Congenital Abnormalities
 Thyroid Diseases Antibodies  >1 publications with IL-33 and Thyroid Diseases
 Skin Neoplasms Antibodies  >1 publications with IL-33 and Skin Neoplasms

Organs/Tissues associated with IL-33 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with IL-33 and Blood
 Thymus Antibodies  >4 publications with IL-33 and Thymus
 Brain Antibodies  >4 publications with IL-33 and Brain
 Vascular Antibodies  >3 publications with IL-33 and Vascular
 Eye Antibodies  >3 publications with IL-33 and Eye
 Testis Antibodies  >2 publications with IL-33 and Testis
 Spinal Cord Antibodies  >1 publications with IL-33 and Spinal Cord
 Bone Antibodies  >1 publications with IL-33 and Bone
 Skin Antibodies  >1 publications with IL-33 and Skin
 Embryonic Tissue Antibodies  >1 publications with IL-33 and Embryonic Tissue
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