Granulocyte-Colony Stimulating Factor (G-CSF) Human Recombinant Protein

G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is produced also by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM-CSF.Comparison of the primary sequence of G-CSF with those of the two other colony stimulating factors, GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF show a sequence homology of approximately 70 % at the DNA level and of 72 % at the protein level. The G-CSF receptor,CD114, is expressed on all cells of the neutrophils and granulocytes lineage. It is expressed also in placenta cells, endothelial cells and various carcinoma cell lines.Human G-CSF is active in murine cells and vice versa. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner. G-CSF synergises with some other cytokines, including GM-CSF and IL4. GM-CSF and G-CSF are required, for example, to develop neutrophilic colonies in vitro. The concerted action of G-CSF and Epo is required to support the growth of mixed colonies of the early erythroid progenitors. A combination of IL4 with G-CSF has been shown to lead to synergistic suppression of the growth of some human leukemic cell lines.

• plerixafor (amd3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different cd34+ cell populations based on global gene and microrna expression signatures .Blood, Sep 2009; 114: 2530 - 2541.
• the role of granulocyte-colony stimulating factor (G-CSF) in the healthy brain: a characterization of G-CSF-deficient mice .J. Neurosci., Sep 2009; 29: 11572 - 11581.
• nfi-a directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls β-globin and G-CSF receptor expression .Blood, Aug 2009; 114: 1753 - 1763.
• silencing of microrna-155 in mice during acute inflammatory response leads to derepression of c/ebp beta and down-regulation of G-CSF .Nucleic Acids Res., Jul 2009; 10.1093/nar/gkp577.
• preoperative weekly cisplatin–epirubicin–paclitaxel with G-CSF support in triple-negative large operable breast cancer .Ann. Onc., Jul 2009; 20: 1185 - 1192.