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Erythropoietin-Alpha (EPO) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553224

EPO is predominantly synthesized and secreted by tubular and juxtatubular capillary endothelial and interstitial cells of the kidney. Approximately 10-15 % of the total amount of Epo comes from extrarenal sources and is predominantly produced by hepatocytes… More details

$240 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Erythropoietin-alpha (EPO), Recombinant Protein
Full Product Name
Human Erythropoietin-alpha Recombinant
Product Gene Name
EPO recombinant protein
Product Synonym Names
epo; Erythropoietin
Sequence
MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE NITTGCAEHC SLNENITVPD TKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQALLVN SSQPWEPLQL HVDKAVSGLR SLTTLLRALG AQKEAISPPD AASAAPLRTI TADTFRKLFR VYSNFLRGKL KLYTGEACRT GDR
Host
Chinese Hamster Ovary Cells (CHO) (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant Erythropoietin alpha was lyophilized from a 0.2 um filtered PBS solution pH 7.
Purity/Purification
>98%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
21,307 Da
OMIM
133170
3D Structure
ModBase 3D Structure for P01588
Domain
4 helix cytokine core
Host Note
Optimized DNA sequence encoding Human EPO mature chain was expressed in CHO cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Molecular Weight Note
Native human Erythropoietin-alpha is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20 kDa.

Recombinant EPO-a is a glycosylated disulfide-linked homodimeric protein consisting of 166 amino acid residue subunits, and migrates as an approximately 35 kDa protein under reducing conditions in SDS-PAGE.

Product Categories/Family for EPO recombinant protein

Cytokines

Other Notes

Small volumes of EPO recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPO purchase

MBS553224 is a recombinant protein targeting EPO. Also known as Human Erythropoietin-alpha Recombinant. Host: Chinese Hamster Ovary Cells (CHO) (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for EPO. It may not necessarily be applicable to this product.

NCBI GI #
62240997
NCBI GeneID
2056
NCBI Accession #
NP_000790.2
UniProt Primary Accession #
P01588
UniProt Secondary Accession #
Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0
UniProt Related Accession #
P01588
GenBank Nucleotide #
NM_000799.2
NCBI Official Full Name
erythropoietin
UniProt Protein Name
Erythropoietin
Protein Family
Erythropoietin
UniProt Gene Name
EPO
UniProt Entry Name
EPO_HUMAN

NCBI Summary for EPO

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]
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UniProt Comments for EPO

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: protein binding; erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; erythrocyte maturation; blood circulation; apoptosis; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to testosterone stimulus; signal transduction; response to salt stress; response to vitamin A; peptidyl-serine phosphorylation; response to hyperoxia; positive regulation of tyrosine phosphorylation of Stat5 protein; response to estrogen stimulus; positive regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of Ras protein signal transduction; response to axon injury; response to electrical stimulus; positive regulation of neuron differentiation; positive regulation of DNA replication; embryo implantation; aging

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EPO recombinant protein

 Reference Product  PubMed Publications
 EPOR recombinant protein  >566 publications with EPO and EPOR
 HIF1A recombinant protein  >193 publications with EPO and HIF1A
 RB1 recombinant protein  >1 publications with EPO and RB1

Pathways associated with EPO recombinant protein

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 EPO Signaling Pathway antibodies  EPO Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram

Diseases associated with EPO recombinant protein

 Disease Name  Pubmed Publications
 Anemia Antibodies  >2385 publications with EPO and Anemia
 Kidney Diseases Antibodies  >1183 publications with EPO and Kidney Diseases
 Neoplasms Antibodies  >1061 publications with EPO and Neoplasms
 Renal Insufficiency Antibodies  >882 publications with EPO and Renal Insufficiency
 Kidney Failure, Chronic Antibodies  >844 publications with EPO and Kidney Failure, Chronic
 Leukemia Antibodies  >546 publications with EPO and Leukemia
 Inflammation Antibodies  >426 publications with EPO and Inflammation
 Central Nervous System Diseases Antibodies  >360 publications with EPO and Central Nervous System Diseases
 Heart Diseases Antibodies  >279 publications with EPO and Heart Diseases
 Hypertension Antibodies  >270 publications with EPO and Hypertension

Organs/Tissues associated with EPO recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >586 publications with EPO and Brain
 Eye Antibodies  >106 publications with EPO and Eye
 Prostate Antibodies  >26 publications with EPO and Prostate
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