SARS E Recombinant Protein – Cat# MBS553225

SARS Envelope Protein (SARS E) Recombinant Protein

Name: SARS E Recombinant Protein; MyBioSource
Brand: MyBioSource
SKU: MBS553225
Price: 685 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553225

MBS553225 is a recombinant protein targeting SARS E. Also known as SARS Envelope protein Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

$685 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SARS Envelope protein, Recombinant Protein

Full Product Name

SARS Envelope protein Recombinant

Product Gene Name

SARS E recombinant protein

Product Synonym Names

sars; envelope

Host

E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)

Form/Format

Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.

Purity/Purification

>95%, as determined by SDS-PAGE.

Preparation and Storage

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

Molecular Weight

8,361 Da

3D Structure

ModBase 3D Structure for P59637

Host Note

DNA sequence encoding immunodominant fragment of SARS Envelope protein was expressed in Escherichia Coli.

Reconstitution

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Molecular Weight Note

Recombinant SARS E encodes the immunodominant sequences of the SARS Envelope small membrane protein.

Recombinant SARS E contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Product Categories/Family for SARS E recombinant protein

Viral Antigens

Application Notes for SARS E recombinant protein

SARS antigen is suitable for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems.

Other Notes

Small volumes of SARS E recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SARS E. It may not necessarily be applicable to this product.

NCBI GI #

29836499

NCBI GeneID

1489671

NCBI Accession #

NP_828854.1

UniProt Primary Accession #

P59637

UniProt Secondary Accession #

Q7TA13; Q7TFA8

GenBank Nucleotide #

NC_004718.3

NCBI Official Full Name

protein E

UniProt Protein Name

Envelope small membrane protein

UniProt Gene Name

UniProt Synonym Gene Names

sM; E protein; sM protein

UniProt Entry Name

VEMP_CVHSA

UniProt Comments for SARS E

Function: Component of the viral envelope that plays a central role in virus morphogenesis and assembly. It is sufficient to form virus-like particles. Seems to be important for creating the membrane curvature needed to acquire the rounded, stable and infectious particle phenotype. Acts as a viroporin, inducing the formation of hydrophilic pores in cellular membranes. Also induces apoptosis

By similarity.

Subunit structure: Homooligomer. Interacts with the M membrane protein in the budding compartment of the host cell, which is located between endoplasmic reticulum and the Golgi complex

By similarity. Interacts with the accessory proteins 3a and 7a. Ref.21 Ref.22 Ref.23

Subcellular location: Virion membrane; Single-pass type III membrane protein

Potential. Host Golgi apparatus membrane; Single-pass type III membrane protein

Potential. Note: Largely membrane-embedded. The N-terminus could be located within the membrane near the cytoplasmic side since no part of the protein is aparently exposed on the virion outside. The large hydrophobic domain could form a hairpin, looping back through the membrane. There also may be a second hydrophobic domain integrated into membranes

By similarity.

Sequence similarities: Belongs to the coronaviruses E protein family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SARS E recombinant protein

Reference Product	PubMed Publications
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