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Prolactin (PRL) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553238

Prolactin is a neuroendocrine pituitary hormone of 23 kDa. It is produced in increasing amounts during pregnancy and during suckling and acts primarily on the mammary gland by initiating and maintaining lactation in the postpartal phase. rolactin has been… More details

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Product Overview

Online Datasheet in PDF
Product Name
Prolactin (PRL), Recombinant Protein
Full Product Name
Mouse Prolactin recombinant
Product Gene Name
PRL recombinant protein
Product Synonym Gene Name
Prolactin
Product Synonym Names
prl; prolactin
Sequence
MNSQGSAQKA GTLLLLLISN LLFCQNVQPL PICSAGDCQT SLRELFDRVV ILSHYIHTLY TDMFIEFDKQ YVQDREFMVK VINDCPTSSL ATPEDKEQAL KVPPEVLLNL ILSLVQSSSD PLFQLITGVG GIQEAPEYIL SRAKEIEEQN KQLLEGVEKI ISQAYPEAKG NGIYFVWSQL PSLQGVDEES KILSLRNTIR CLRRDSHKVD NFLKVLRCQI AHQNNC
Host
Insect Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant mouse Prolactin is presented as concentrated 1mg/ml, 0.2 um filtered 20mM Tris solution,0.5M NaCl pH 7.4 containing 5% trehalose 10% glycerol.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
25,496 Da
3D Structure
ModBase 3D Structure for P06879
Domain
four helix cytokine core
Host Note
Optimized DNA sequence encoding Mouse Prolactin mature chain fused to a C terminal polyHis tag was expressed in Insect Cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration of 0.1 mg/mL.
Molecular Weight Note
Native mouse Prolactin is generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 23 kDa.

Recombinant Prolcatin is a disulfide-linked monomeric protein consisting of 207 amino acid residue subunits, and migrates as an approximately 26 kDa protein under non-reducing conditions in SDS-PAGE.

Product Categories/Family for PRL recombinant protein

Cytokines

Other Notes

Small volumes of PRL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRL purchase

MBS553238 is a recombinant protein targeting PRL. Also known as Mouse Prolactin recombinant. Host: Insect Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for PRL. It may not necessarily be applicable to this product.

NCBI GI #
130934
NCBI GeneID
19109
NCBI Accession #
P06879.1
UniProt Primary Accession #
P06879
UniProt Secondary Accession #
Q544X8
NCBI Official Full Name
Prolactin
UniProt Protein Name
Prolactin
Protein Family
Prolactin
UniProt Gene Name
Prl
UniProt Synonym Gene Names
PRL
UniProt Entry Name
PRL_MOUSE

UniProt Comments for PRL

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Secreted; Secreted, signal peptide; Cytokine; Motility/polarity/chemotaxis

Cellular Component: extracellular region; secretory granule

Molecular Function: prolactin receptor binding; hormone activity

Biological Process: lactation; regulation of JAK-STAT cascade; mammary gland development; maternal behavior; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; regulation of ossification; positive regulation of epithelial cell proliferation; STAT protein nuclear translocation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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