Rat CXCL3 Recombinant Protein – Cat# MBS553260

GRO-Gamma Alpha (CXCL3) Rat Recombinant Protein

Name: CXCL3 Recombinant Protein; GRO-Gamma Alpha; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS553260
Price: 240 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553260

Growth regulated oncogene- gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity) and the new designation is CXCL3. This factor is known mainly because of its chemotactic… More details

$240 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GRO-gamma alpha (CXCL3), Recombinant Protein

Full Product Name

Rat GRO-gamma alpha (CXCL3) Recombinant

Product Gene Name

CXCL3 recombinant protein

Product Synonym Gene Name

GRO-gamma

Product Synonym Names

Rat GRO-g recombinant; GRO-gamma; GROg; cxcl3; mip2; mip-2

Sequence

MGEIRVDNFS LEDFFSGDID SYNYSSDPPF TLSDAAPCPS ANLDINRYAV VVIYVLVTLL SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WIFGSFLCKV FSFLQEITFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCITMWF LSLVLSLPIF ILRTTVKANP STVVCYENIG NNTSKWRVVL RILPQTYGFL LPLLIMLFCY GFTLRTLFKA HMGQKHRAMR VIFAVVLVFL LCWLPYNIVL FTDTLMRTKL IKETCERQNE INKALEATEI LGFLHSCLNP IIYAFIGQKF RHGLLKIMAN YGLVSKEFLA KEGRPSFVGS SSANTSTTL

Host

E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)

Form/Format

Recombinant mouse GRO-gamma/CXCL3 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 40mM NaCl, 10mM PB, pH 7.0.

Purity/Purification

>95%, as determined by SDS-PAGE and HPLC

Preparation and Storage

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

Molecular Weight

11,109 Da

3D Structure

ModBase 3D Structure for Q10746

Domain

IL8

Host Note

Optimized DNA sequence encoding rat GRO-gamma/CXCL3 mature chain was expressed in Escherichia Coli

Endotoxin

Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).

Reconstitution

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Molecular Weight Note

Native rat GROgamma/CXCL3 is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa.

Recombinant GRO-gamma is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for CXCL3 recombinant protein

Cytokines

Other Notes

Small volumes of CXCL3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXCL3 purchase

MBS553260 is a recombinant protein targeting CXCL3. Also known as Rat GRO-gamma alpha (CXCL3) Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for CXCL3. It may not necessarily be applicable to this product.

NCBI GI #

19924051

NCBI GeneID

171551

NCBI Accession #

NP_612531.1

UniProt Primary Accession #

Q10746

UniProt Secondary Accession #

Q10747; Q5CZ55; Q9EP62

GenBank Nucleotide #

NM_138522.1

NCBI Official Full Name

C-X-C motif chemokine 3

UniProt Protein Name

C-X-C motif chemokine 3

UniProt Synonym Protein Names

Cytokine-induced neutrophil chemoattractant 2; CINC-2; Macrophage inflammatory protein 2-alpha/beta

Protein Family

C-X-C motif chemokine

UniProt Gene Name

Cxcl3

UniProt Synonym Gene Names

Cinc2; CINC-2

UniProt Entry Name

CXCL3_RAT

NCBI Summary for CXCL3

acts as a chemoattractant for neutrophils; involved in the inflammatory response [RGD, Feb 2006]

UniProt Comments for CXCL3

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; hemopoiesis; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; immune response; inflammatory response; chemotaxis; regulation of cell proliferation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.