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MCP-5 (CCL12) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553324

MCP5/CCL12 is a Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a… More details

$240 / 0.005 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MCP-5 (CCL12), Recombinant Protein
Full Product Name
Mouse MCP-5 (CCL12) Recombinant
Product Gene Name
CCL12 recombinant protein
Product Synonym Gene Name
MCP-5
Product Synonym Names
Mouse MCP-5 recombinant; mcp-5; ccl12; mcp5; ccl-12
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant MCP-5 was lyophilized from a 0.2 um filtered 20mM PB solution pH 7.5, 100mM NaCl.
Purity/Purification
>98%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
11,659 Da
3D Structure
ModBase 3D Structure for Q62401
Domain
IL8
Host Note
Optimized DNA sequence encoding Mouse MCP-5 (CCL12) mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Mouse MCP-5/CCL12, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 10kDa.

Recombinant MCP-5/CCL12 is a disulfide-linked monomeric protein consisting of 83 amino acid residue subunits and migrates as an approximately 10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Product Categories/Family for CCL12 recombinant protein

Cytokines

Other Notes

Small volumes of CCL12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL12 purchase

MBS553324 is a recombinant protein targeting CCL12. Also known as Mouse MCP-5 (CCL12) Recombinant. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for CCL12. It may not necessarily be applicable to this product.

NCBI GI #
119226206
NCBI GeneID
20293
NCBI Accession #
NP_035461.2
UniProt Primary Accession #
Q62401
UniProt Secondary Accession #
Q9QYD6
GenBank Nucleotide #
NM_011331.2
NCBI Official Full Name
C-C motif chemokine 12
UniProt Protein Name
C-C motif chemokine 12
UniProt Synonym Protein Names
MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; MCP-5; Small-inducible cytokine A12
Protein Family
C-C motif chemokine
UniProt Gene Name
Ccl12
UniProt Synonym Gene Names
Mcp5; Scya12; MCP-5
UniProt Entry Name
CCL12_MOUSE

UniProt Comments for CCL12

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: CCR2 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: positive regulation of leukocyte migration; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; chemotaxis; macrophage chemotaxis; monocyte chemotaxis; regulation of cell shape; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; immune response; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; astrocyte cell migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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