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THBD Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553648

MBS553648 is an active protein targeting THBD. Host: CHO (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
THBD, Active Protein
Full Product Name
THBD
Product Gene Name
THBD active protein
Sequence
APAEPQPGGS QCVEHDCFAL YPGPATFLNA SQICDGLRGH LMTVRSSVAA DVISLLLNGD GGVGRRRLWI GLQLPPGCGD PKRLGPLRGF QWVTGDNNTS YSRWARLDLN GAPLCGPLCV AVSAAEATVP SEPIWEEQQC EVKADGFLCE FHFPATCRPL AVEPGAAAAA VSITYGTPFA ARGADFQALP VGSSAAVAPL GLQLMCTAPP GAVQGHWARE APGAWDCSVE NGGCEHACNA IPGAPRCQCP AGAALQADGR SCTASATQSC NDLCEHFCVP NPDQPGSYSC MCETGYRLAA DQHRCEDVDD CILEPSPCPQ RCVNTQGGFE CHCYPNYDLV DGECVEPVDP CFRANCEYQC QPLNQTSYLC VCAEGFAPIP HEPHRCQMFC NQTACPADCD PNTQASCECP EGYILDDGFI CTDIDECENG GFCSGVCHNL PGTFECICGP DSALARHIGT DCDSGKVDGG DSGSGEPPPS PTPGSTLTPP A
Host
CHO (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized; PBS, pH 7.2
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
60,329 Da
3D Structure
ModBase 3D Structure for P07204
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Source Note
Optimized DNA sequence encoding Human THBD mature chain was expressed in CHO. Recombinant Human Thrombomodulin encodes amino acid residues 19-575.
Biological Activity
Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is greater than 500 pmoles/min/ug.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Applications Tested/Suitable for THBD active protein
SDS-PAGE
Application Notes for THBD active protein
Recombinant THBD migrates as an approximately 54 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.

Other Notes

Small volumes of THBD active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for THBD. It may not necessarily be applicable to this product.

NCBI GI #
4507483
NCBI GeneID
7056
NCBI Accession #
NP_000352.1
UniProt Primary Accession #
P07204
UniProt Secondary Accession #
Q8IV29; Q9UC32
UniProt Related Accession #
P07204
GenBank Nucleotide #
NM_000361.2
NCBI Official Full Name
thrombomodulin
NCBI Official Synonym Full Names
thrombomodulin
NCBI Official Symbol
THBD
NCBI Official Synonym Symbols
TM; THRM; AHUS6; BDCA3; CD141; THPH12
UniProt Protein Name
Thrombomodulin
UniProt Synonym Protein Names
Fetomodulin; CD_antigen: CD141
Protein Family
Thrombomodulin
UniProt Gene Name
THBD
UniProt Synonym Gene Names
THRM; TM
NCBI Protein Information
thrombomodulin

NCBI Summary for THBD

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]
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UniProt Comments for THBD

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
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Research Articles on THBD

1. Thrombomodulin gene C1418T polymorphism is associated with Kawasaki disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with THBD active protein

 Reference Product  PubMed Publications
 PROC active protein  >2 publications with THBD and PROC

Pathways associated with THBD active protein

 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway antibodies  Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway Diagram

Diseases associated with THBD active protein

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >31 publications with THBD and Cardiovascular Diseases
 Thrombosis Antibodies  >20 publications with THBD and Thrombosis
 Inflammation Antibodies  >19 publications with THBD and Inflammation
 Neoplasms Antibodies  >18 publications with THBD and Neoplasms
 Kidney Diseases Antibodies  >14 publications with THBD and Kidney Diseases
 Thrombophilia Antibodies  >13 publications with THBD and Thrombophilia
 Disseminated Intravascular Coagulation Antibodies  >10 publications with THBD and Disseminated Intravascular Coagulation
 Heart Diseases Antibodies  >8 publications with THBD and Heart Diseases
 Atypical hemolytic uremic syndrome Antibodies  >7 publications with THBD and Atypical hemolytic uremic syndrome
 Nervous System Diseases Antibodies  >6 publications with THBD and Nervous System Diseases

Organs/Tissues associated with THBD active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >63 publications with THBD and Blood
 Vascular Antibodies  >28 publications with THBD and Vascular
 Liver Antibodies  >9 publications with THBD and Liver
 Kidney Antibodies  >9 publications with THBD and Kidney
 Lung Antibodies  >8 publications with THBD and Lung
 Bone Antibodies  >7 publications with THBD and Bone
 Brain Antibodies  >6 publications with THBD and Brain
 Embryonic Tissue Antibodies  >6 publications with THBD and Embryonic Tissue
 Stomach Antibodies  >5 publications with THBD and Stomach
 Placenta Antibodies  >4 publications with THBD and Placenta
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