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WNT3A Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553660

MBS553660 is an active protein targeting WNT3A. Host: CHO (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WNT3A, Active Protein
Full Product Name
WNT3A
Product Gene Name
WNT3A active protein
Sequence
SYPIWWSLAV GPQYSSLSTQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GVKAGIQECQ HQFRGRRWNC TTVSNSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGSAAICGC SSRLQGSPGE GWKWGGCSED IEFGGMVSRE FADARENRPD ARSAMNRHNN EAGRQAIASH MHLKCKCHGL SGSCEVKTCW WSQPDFRTIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRP RYTYFKVPTE RDLVYYEASP NFCEPNPETG SFGTRDRTCN VSSHGIDGCD LLCCGRGHNA RTERRREKCH CVFHWCCYVS CQECTRVYDV HTCK
Host
CHO (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized; PBS, pH 7.2
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
42,905 Da
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Source Note
Optimized DNA sequence encoding Mouse WNT3A mature chain including a C-terminal 6xHis tag was expressed in CHO. Recombinant Mouse Protein Wnt-3a encodes amino acid residues 19-352.
Biological Activity
Determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 for this effect is 0.4-0.6 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Applications Tested/Suitable for WNT3A active protein
SDS-PAGE
Application Notes for WNT3A active protein
Recombinant WNT3A migrates as an approximately 37 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.

Other Notes

Small volumes of WNT3A active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WNT3A. It may not necessarily be applicable to this product.

NCBI GI #
14530679
NCBI GeneID
89780
NCBI Accession #
BAB61052.1
UniProt Secondary Accession #
Q3SY79; Q3SY80; Q969P2
UniProt Related Accession #
P56704
NCBI Official Full Name
WNT3A
NCBI Official Synonym Full Names
Wnt family member 3A
NCBI Official Symbol
WNT3A
UniProt Protein Name
Protein Wnt-3a
UniProt Gene Name
WNT3A
NCBI Protein Information
protein Wnt-3a

NCBI Summary for WNT3A

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
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UniProt Comments for WNT3A

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).
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Research Articles on WNT3A

1. The miR-766-3p suppressed hepatocellular carcinoma (HCC) cell growth and invasion via a Wnt3a/PRC1 positive regulatory loop, and miR-766-3p may serve as a potential therapeutic target in HCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WNT3A active protein

 Reference Product  PubMed Publications
 CTNNB1 active protein  >561 publications with WNT3A and CTNNB1
 DKK1 active protein  >106 publications with WNT3A and DKK1
 LRP6 active protein  >77 publications with WNT3A and LRP6
 FZD1 active protein  >19 publications with WNT3A and FZD1
 AXIN1 active protein  >12 publications with WNT3A and AXIN1
 DVL3 active protein  >12 publications with WNT3A and DVL3
 FZD8 active protein  >8 publications with WNT3A and FZD8
 WIF1 active protein  >6 publications with WNT3A and WIF1
 DVL1 active protein  >5 publications with WNT3A and DVL1

Pathways associated with WNT3A active protein

 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram

Diseases associated with WNT3A active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >140 publications with WNT3A and Neoplasms
 Skin Diseases Antibodies  >29 publications with WNT3A and Skin Diseases
 Congenital Abnormalities Antibodies  >24 publications with WNT3A and Congenital Abnormalities
 Breast Neoplasms Antibodies  >23 publications with WNT3A and Breast Neoplasms
 Necrosis Antibodies  >21 publications with WNT3A and Necrosis
 Nervous System Diseases Antibodies  >20 publications with WNT3A and Nervous System Diseases
 Fibrosis Antibodies  >19 publications with WNT3A and Fibrosis
 Inflammation Antibodies  >17 publications with WNT3A and Inflammation
 Heart Diseases Antibodies  >13 publications with WNT3A and Heart Diseases
 Liver Diseases Antibodies  >13 publications with WNT3A and Liver Diseases

Organs/Tissues associated with WNT3A active protein

 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >40 publications with WNT3A and Connective Tissue
 Lung Antibodies  >28 publications with WNT3A and Lung
 Placenta Antibodies  >9 publications with WNT3A and Placenta
 Testis Antibodies  >5 publications with WNT3A and Testis
 Trachea Antibodies  >1 publications with WNT3A and Trachea
 Uncharacterized Tissue Antibodies  >1 publications with WNT3A and Uncharacterized Tissue
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