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IL15 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553664

MBS553664 is an active protein targeting IL15. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IL15, Active Protein
Full Product Name
IL15
Product Gene Name
IL15 active protein
Sequence
MKILKPYMRN TSISCYLCFL LNSHFLTEAG IHVFILGCVS VGLPKTEANW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized; 0.2 um filtered PBS solution
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
14,912 Da
3D Structure
ModBase 3D Structure for P40933
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Source Note
Optimized DNA sequence encoding mouse Interleukin-15 mature chain was expressed in Escherichia Coli.
Biological Activity
The ED50 was determined by the dose-dependent proliferation of murine CTLL-2 cells is < 2.0 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Applications Tested/Suitable for IL15 active protein
SDS-PAGE
Application Notes for IL15 active protein
Native mouse Interleukin-15 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant mouse Interleukin-15 is a disulfide-linked homodimeric protein consisting of 115 amino acid residue subunits, and migrates as an approximately 13kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Other Notes

Small volumes of IL15 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL15. It may not necessarily be applicable to this product.

NCBI GI #
10835153
NCBI GeneID
3600
NCBI Accession #
NP_000576.1
UniProt Primary Accession #
P40933
UniProt Secondary Accession #
O00440; O43512; Q495Z8; Q6FGX7; Q93058; Q9UBA3; D3DNZ2
UniProt Related Accession #
P40933
GenBank Nucleotide #
NM_000585.5
NCBI Official Full Name
interleukin-15 isoform 1 preproprotein
NCBI Official Synonym Full Names
interleukin 15
NCBI Official Symbol
IL15
NCBI Official Synonym Symbols
IL-15
UniProt Protein Name
Interleukin-15
Protein Family
Interleukin
UniProt Gene Name
IL15
UniProt Synonym Gene Names
IL-15
NCBI Protein Information
interleukin-15

NCBI Summary for IL15

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]
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UniProt Comments for IL15

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

Research Articles on IL15

1. High IL15 expression is associated with follicles with immature oocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL15 active protein

 Reference Product  PubMed Publications
 IL15RA active protein  >227 publications with IL15 and IL15RA
 PRF1 active protein  >101 publications with IL15 and PRF1
 IL2RA active protein  >100 publications with IL15 and IL2RA
 IL2RB active protein  >81 publications with IL15 and IL2RB
 IL2RG active protein  >70 publications with IL15 and IL2RG
 IL7 active protein  >31 publications with IL15 and IL7
 IL4 active protein  >20 publications with IL15 and IL4
 IFNG active protein  >8 publications with IL15 and IFNG

Pathways associated with IL15 active protein

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram

Diseases associated with IL15 active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >449 publications with IL15 and Neoplasms
 Necrosis Antibodies  >318 publications with IL15 and Necrosis
 Inflammation Antibodies  >303 publications with IL15 and Inflammation
 Disease Models, Animal Antibodies  >150 publications with IL15 and Disease Models, Animal
 Death Antibodies  >130 publications with IL15 and Death
 Nervous System Diseases Antibodies  >93 publications with IL15 and Nervous System Diseases
 Lung Diseases Antibodies  >90 publications with IL15 and Lung Diseases
 Neoplasms, Experimental Antibodies  >88 publications with IL15 and Neoplasms, Experimental
 Hematologic Diseases Antibodies  >80 publications with IL15 and Hematologic Diseases
 Liver Diseases Antibodies  >51 publications with IL15 and Liver Diseases

Organs/Tissues associated with IL15 active protein

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >40 publications with IL15 and Mixed
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