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MSLN Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553684

MBS553684 is a recombinant protein targeting MSLN. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MSLN, Recombinant Protein
Full Product Name
MSLN
Product Gene Name
Sequence
LAGETGQEAA PLDGVLANPP NISSLSPRQL LGFPCAEVSG LSTERVRELA VALAQKNVKL STEQLRCLAH RLSEPPEDLD ALPLDLLLFL NPDAFSGPQA CTRFFSRITK ANVDLLPRGA PERQRLLPAA LACWGVRGSL LSEADVRALG GLACDLPGRF VAESAEVLLP RLVSCPGPLD QDQQEAARAA LQGGGPPYGP PSTWSVSTMD ALRGLLPVLG QPIIRSIPQG IVAAWRQRSS RDPSWRQPER
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized; PBS, pH 7.2
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
68,070 Da
3D Structure
ModBase 3D Structure for Q13421
Species
Human
Source Note
Optimized DNA sequence encoding Human MSLN mature chain was expressed in ECOLI. Recombinant Human Mesothelin encodes amino acid residues 37-606.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Applications Tested/Suitable for MSLN recombinant protein
SDS-PAGE
Application Notes for MSLN recombinant protein
Recombinant MSLN migrates as an approximately 28 kDa protein under reducing conditions in SDS-PAGE.

Other Notes

Small volumes of MSLN recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MSLN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
UniProt Protein Name
Mesothelin
UniProt Synonym Protein Names
CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
Protein Family
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
NCBI Protein Information
mesothelin

NCBI Summary for MSLN

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
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UniProt Comments for MSLN

Membrane-anchored forms may play a role in cellular adhesion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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