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GDF2 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553714

MBS553714 is an active protein targeting GDF2. Host: CHO (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
GDF2, Active Protein
Full Product Name
GDF2
Product Gene Name
GDF2 active protein
Sequence
SAGAGSHCQK TSLRVNFEDI GWDSWIIAPK E YEAYECKGGC FFPLADDVTP TK HAIVQTLVHL KFPTKVGKAC CVPTKLSPIS VLYKDDMGVP TLKYHYEGMS VAECGCR
Host
CHO (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized; PBS, pH 7.2
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
47,320 Da
3D Structure
ModBase 3D Structure for Q9UK05
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Source Note
Optimized DNA sequence encoding Human GDF2 mature chain was expressed in CHO. Recombinant Human Growth/differentiation factor 2 encodes amino acid residues 320-429.
Biological Activity
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Applications Tested/Suitable for GDF2 active protein
SDS-PAGE
Application Notes for GDF2 active protein
Recombinant GDF2 migrates as an approximately 12 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.

Other Notes

Small volumes of GDF2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GDF2. It may not necessarily be applicable to this product.

NCBI GI #
7705308
NCBI GeneID
2658
NCBI Accession #
NP_057288.1
UniProt Primary Accession #
Q9UK05
UniProt Secondary Accession #
Q5VSQ9; Q9Y571
UniProt Related Accession #
Q9UK05
GenBank Nucleotide #
NM_016204.3
NCBI Official Full Name
growth/differentiation factor 2 preproprotein
NCBI Official Synonym Full Names
growth differentiation factor 2
NCBI Official Symbol
GDF2
NCBI Official Synonym Symbols
BMP9; HHT5; BMP-9
UniProt Protein Name
Growth/differentiation factor 2
UniProt Synonym Protein Names
Bone morphogenetic protein 9; BMP-9
Protein Family
Growth/differentiation factor
UniProt Gene Name
GDF2
UniProt Synonym Gene Names
BMP9; GDF-2; BMP-9
NCBI Protein Information
growth/differentiation factor 2

NCBI Summary for GDF2

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. [provided by RefSeq, Jul 2016]
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UniProt Comments for GDF2

Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

Research Articles on GDF2

1. Case-control analyses reveal significant overrepresentation of rare variants in ATP13A3, AQP1 and SOX17, and provide independent validation of a critical role for GDF2 in heritable pulmonary arterial hypertension.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GDF2 active protein

 Reference Product  PubMed Publications
 ACVRL1 active protein  >15 publications with GDF2 and ACVRL1
 BMPR2 active protein  >13 publications with GDF2 and BMPR2
 ENG active protein  >10 publications with GDF2 and ENG
 SMAD5 active protein  >4 publications with GDF2 and SMAD5
 HEY1 active protein  >3 publications with GDF2 and HEY1
 CHAT active protein  >2 publications with GDF2 and CHAT
 HAMP active protein  >1 publications with GDF2 and HAMP
 SMAD9 active protein  >1 publications with GDF2 and SMAD9

Pathways associated with GDF2 active protein

 Products by Pathway  Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram

Diseases associated with GDF2 active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with GDF2 and Neoplasms
 Congenital Abnormalities Antibodies  >4 publications with GDF2 and Congenital Abnormalities
 Disease Models, Animal Antibodies  >3 publications with GDF2 and Disease Models, Animal
 Lung Diseases Antibodies  >1 publications with GDF2 and Lung Diseases
 Drug Toxicity Antibodies  >1 publications with GDF2 and Drug Toxicity
 Intestinal Diseases Antibodies  >1 publications with GDF2 and Intestinal Diseases
 Inflammation Antibodies  >1 publications with GDF2 and Inflammation

Organs/Tissues associated with GDF2 active protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >6 publications with GDF2 and Liver
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