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ACE-2 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553727

MBS553727 is an active protein targeting ACE-2. Also known as cynomolgus ACE2 Fc. Host: HEK Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ACE-2, Active Protein
Full Product Name
cynomolgus ACE2 Fc
Product Gene Name
ACE-2 active protein
Host
HEK Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant COVID-19 Spike S1 was lyophilized from 0.2 um filtered PBS solution pH7.4
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source Note
DNA sequence encoding extracellular fragment[16-675] of NCOV Spike S1 [accession# QIC53204.1] including a C terminal polyHis was expressed in HEK cells.
Activity
The activity was tested by binding Human ACE2-Fc in functional ELISA assay, the calculated EC50 was determined to be 0.1ug/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

SDS-Page of ACE-2 active protein

The recombinant S1-His protein migrates as 120kDa due to glycosylation
ACE-2 Active Protein – Cat# MBS553727

Biological Activity of ACE-2 active protein

2019-ncov S1-his tagged (coating at 0.5ug-well) binding with Human ACE2-Fc. The linear range was found to be 0.1-5 ug/ml
ACE-2 Active Protein, Biological Activity – Cat# MBS553727

Other Notes

Small volumes of ACE-2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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