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COVID 19 Spike S1 Coronavirus (COVID-19) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553728

Recombinant NCOV1 S1 encodes the NCOV spike glycoprotein S1 sequence. This molecule has a calculated mass of approximately 100kDa. Recombinant S1 contains an C-Terminal polyHis purification tag and migrates due to glycosylation as an approximately 120 kDa… More details

$520 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COVID 19 Spike S1 Coronavirus (COVID-19), Active Protein
Full Product Name
2019 nCoV/COVID19 Spike protein S1 Recombinant-His
Product Gene Name
COVID-19 active protein
Product Synonym Gene Name
2019-nCoV; SARS-CoV-2; SARS coronavirus 2 proteome; COVID19; SARS-CoV2
Product Synonym Names
2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike protein S1 His
Host
N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized from sterile solution of pH7.4
Purity/Purification
> 90% by SDS-PAGE.
Preparation and Storage
Lyophilized store at -20°C for 24 months.
Lyophilized store at ambient temperature for 3 weeks.
Reconstituted store at -20°C for 12 months.
Reconstituted store at 2-8°C for 3 weeks.
For long term storage we recommend the addition of carrier protein at 0.1%. Avoid repeated freeze/thaw cycles.
Matching Pairs
Antibody: COVID-19 Clone #001 (MBS553729)
Antigen: COVID-19 (MBS553728)
Comments: The COVID-19 Spike S1 mAb MBS553729 can be used in conjunction with the COVID-19 Spike 1 Recombinant Protein MBS553728 or the Spike RBD Recombinant Protein MBS553719 as a set for ELISA development
Molecular Weight
Recombinant NCOV S1 encodes the NCOV spike glycoprotein S1 sequence . This molecule has a calculated mass of approximately 90kDa. Recombinant S1 migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Host Note
DNA sequence encoding extracellular fragment[16-675] of NCOV Spike S1 [accession# QIC53204.1] was expressed in HEK cells.
Endotoxin
Less than 1EU/ug as determined by LAL method.

SDS-Page of COVID-19 active protein

The recombinant S1-His protein migrates as 120kDa due to glycosylation
COVID-19 Active Protein – Cat# MBS553728

Biological Activity of COVID-19 active protein

The activity was determined by immobilized Spike Protein S1 binding with human ACE2 in a functional ELISA assay, the ED50 was determined to be 3.8nM.

2019-ncov S1-his tagged (coating at 0.5ug-well) binding with Human ACE2-Fc (cat#MBS553725). The linear range was found to be 0.1-5 ug/ml
COVID-19 Active Protein, Biological Activity – Cat# MBS553728

Other Notes

Small volumes of COVID-19 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COVID-19 purchase

MBS553728 is an active protein targeting COVID-19. Also known as 2019 nCoV/COVID19 Spike protein S1 Recombinant-His. Host: N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).
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