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COVID 19 Spike S1 Coronavirus (COVID-19) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553735

MBS553735 is an antibody targeting COVID-19. Also known as Anti SARS-COV2-S1 Monoclonal Antibody, CloneID 003. Reactive in Specific for SARS CoV2 S1 and the RBD domain, has not been tested against other viruses. Host: HEK293 Cells. More details

$465 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COVID 19 Spike S1 Coronavirus (COVID-19), Antibody
Full Product Name
Anti SARS-COV2-S1 Monoclonal Antibody, CloneID 003
Product Gene Name
anti-COVID-19 antibody
Product Synonym Gene Name
2019-nCoV; SARS-CoV-2; SARS coronavirus 2 proteome; COVID19; SARS-CoV2
Product Synonym Names
2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike protein S1
Isotype
Human IgG1 Kappa
Clone Number
003
Host
HEK293 Cells
Species Reactivity
Specific for SARS CoV2 S1 and the RBD domain, has not been tested against other viruses
Form/Format
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.
Purity/Purification
The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Source Note
The recombinant antibody was produced in HEK293 cells. It is a chimera of a mouse variable chain, obtained from mice immunized with the SARS COV2 RBD protein ,fused to human IgG1.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Application Notes for anti-COVID-19 antibody
ELISA: This antibody was validated by ELISA binding to recombinant SARS CoV2 RBD Fc (cat#MBS553719) with a EC50 binding affinity of 0.00447 and to the SARS CoV2 S1 (cat#MBS553728) with a binding affinity of 0.011 (see images below).

SDS-Page of anti-COVID-19 antibody

Reduced SDS PAGE of 10ug loaded sample
Anti-COVID-19 Antibody – Cat# MBS553735

ELISA (EIA) of anti-COVID-19 antibody

Recombinant SARS CoV2 S1-His protein (cat#MBS553728) at 100ng/well binding Anti S1 Monoclonal, with a calculated EC50 of 0.011
Anti-COVID-19 Antibody, ELISA (EIA) – Cat# MBS553735

ELISA (EIA) of anti-COVID-19 antibody

Recombinant SARS CoV2 RBD-Fc protein at 100ng/well binding Anti S1 Monoclonal, with a calculated EC50 of 0.00447
Anti-COVID-19 Antibody, ELISA (EIA) – Cat# MBS553735

Other Notes

Small volumes of anti-COVID-19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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