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COVID 19 Spike Protein S Trimer Coronavirus (COVID-19) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553736

MBS553736 is an active protein targeting COVID-19. Also known as Biotinylated SARS COV2 Spike protein S Trimer Recombinant. Host: HEK Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

$520 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COVID 19 Spike protein S Trimer Coronavirus (COVID-19), Active Protein
Full Product Name
Biotinylated SARS COV2 Spike protein S Trimer Recombinant
Product Gene Name
COVID-19 active protein
Product Synonym Gene Name
2019-nCoV; SARS-CoV-2; SARS coronavirus 2 proteome; COVID19; SARS-CoV2
Product Synonym Names
2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike protein S Trimer
Host
HEK Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 um filtered PBS pH7.4.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C
Molecular Weight
Recombinant 2019-nCoV Spike S trimer protein is a protein consisting of the S1+S2 subunits amino acid residues. The protein has a theoretical MW of 140kDa,however due to glycosylation migrates as an approximately 200 kDa protein on reduced SDS-PAGE.
3D Structure
ModBase 3D Structure for QHD43416
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source Notes
DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1188]R683A (accession # QHD43416.1) fused to a a T4 Folsom trimerzation domain and a C-terminal His AVI tag was expressed in HEK Cells. The furin cleavage site has been mutated at position 683 and 685 so that the protein is not cleaved into the S1 and S2 primary domains by cellular proteases.
Biotinylation
The S trimer is biotinylated in vitro at a single site using the AVItagTM biotinylation technology.
Biological Activity
The activity was tested by binding Human ACE2 in functional ELISA assay (see figure).
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

SDS-Page of COVID-19 active protein

1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
COVID-19 Active Protein – Cat# MBS553736

Activity of COVID-19 active protein

Biotinylated COV2 S trimer(coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The EC50 was determined to be 35 ng/ml
COVID-19 Active Protein, Activity – Cat# MBS553736

Other Notes

Small volumes of COVID-19 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COVID-19. It may not necessarily be applicable to this product.

UniProt Primary Accession #
QHD43416

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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