Fibrillarin/Nop1p Nucleolar Marker Antibody – Mouse

The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiaeNop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein.This protein lacks the RGG richN-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The 3D structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes (Medline link). Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient markerfor nucleoli in a wide variety of species (e.g. 4-6).

Small volumes of anti-Fibrillarin/Nop1p nucleolar marker antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS555050 is an antibody targeting Fibrillarin/Nop1p nucleolar marker. Also known as Fibrillarin/Nop1p nucleolar marker, Monoclonal Antibody, Mouse. Reactive in Human, Mouse, Rat. Host: Mouse.