5-Ht (Serotonin) 7 (5HT7 R) Antibody – Rabbit

Immunohistochemistry: Recommended Dilution: 1/800 - 1/1,000 in PBS/0.3% Triton X-100 - Cy3 technique; 1/10,000 - 1/15,000 in PBS/0.3% Triton X-100 – Biotin/avidin-HRP
Western Blot: Recommended Dilution: 1/1000 or greater
Dilutions listed as a recommendation.
Optimal dilution should be determined by investigator.


Antigen: The histochemical antibody for serotonin (5HT) transporter is generated in a rabbit against a synthetic peptide sequence corresponding to amino acids 579-599 of rat 5HT transporter coupled to KLH. Raised in rabbit.

Control Tissue: The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord.

Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 minutes.

Post Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 minutes.

Post Fixation - 1.5 hour at 4° C. in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.

Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.

Sections: 50 um vibratome

Antibody dilution: 1/300-1/5 in PBS/0.3% Triton X-100 - Bn-SA/HRP Technique

Absorption control: Synthetic rat 5HT Transporter (579 – 599)

Incubation on Tissue: 16-24 hours at 4° C.

Detection System:Use Bn-SA/HRP at dilutions recommended