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mCherry Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS555186

mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent… More details

$520 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
mCherry, Antibody
Full Product Name
mCherry, Affinity Purified Antibody, Rabbit
Product Gene Name
Host
Rabbit
Species Reactivity
All mammalian
Form/Format
Liquid. PBS with 10mM sodium azide as a preservative. Concentration: 1mg/ml.
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Product Type
Affinity Purified
Immunogen Sequence
Recombinant full length His tagged mCherry purified from E. coli.
Applications Tested/Suitable for anti-mCherry antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-mCherry antibody
IF: 1:500
WB: 1:1,000

Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.

Testing Data of anti-mCherry antibody

Image: HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with RPCA-mCherry is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome.
Rabbit Anti-mCherry Antibody – Cat# MBS555186

Western Blot (WB) of anti-mCherry antibody

Image: Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, in the lane marked "+". HEK293 cells which were not transfected with this vector show no protein band in lane marked
Rabbit Anti-mCherry Antibody, Western Blot (WB) – Cat# MBS555186

Other Notes

Small volumes of anti-mCherry antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mCherry purchase

MBS555186 is an antibody targeting mCherry. Also known as mCherry, Affinity Purified Antibody, Rabbit. Reactive in All mammalian. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for mCherry. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
mcherry, partial

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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