Neurotensin (NTS) Antibody – Rabbit

Immunohistochemistry:
- 1:200-1:400 (in PBS/0.3% Triton X-100 – Indirect Immunofluorescence).
- 1:4,000-1:8,000 (in in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique).

Our Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Staining is completely eliminated by pretreatment with 10ug of Neurotensin per 1 mL of diluted antibody.

Tissue Preparation: 10 um cryostat
- Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30 min.
- Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
- Incubation: 18-24 hours at 2-8°C.

Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.