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Vimentin (VIM) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS555481

This protein was produced in E. coli cells and is a single non-glycosylated protein containing 465 amino acids chain and having a molecular mass of 53.5kDa More details

$240 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Recombinant Protein
Full Product Name
Recombinant Human Vimentin
Product Gene Name
VIM recombinant protein
Product Synonym Names
Green fluorescent protein, GFP.
Sequence
STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE
Host
E. coli
Species Reactivity
Human
Form/Format
The protein (1mg/ml) was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride.
Purity/Purification
Greater than 95.0% as determined by bySOS-PAGE.
Preparation and Storage
Lyophilized rHuVim although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuVim should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA).
Please avoid freeze-thaw cycles.
OMIM
116300
Reconstitution
It is recommended to reconstitute the rHuVim in sterile 18MO-cm H20 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Reconstitution to Filaments:
After vimentin is dissolved in 9.5M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Oulbecco's PBS).
Hatzfeld M. and Franke W. W. (1985). J. Cell BioI. 101, 1826-1841
Hatzfeld M. et a/. (1987). J. Mol. BioI. 197, 237-255

Product Categories/Family for VIM recombinant protein

Proteins; Growth/Differentiation Factors and Small Molecules; Cancer; Vimentin

Other Notes

Small volumes of VIM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS555481 is a recombinant protein targeting VIM. Also known as Recombinant Human Vimentin. Reactive in Human. Host: E. coli.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Secondary Accession #
Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.3
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
HEL113; CTRCT30
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
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UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; leading edge; cytoplasm; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; muscle filament sliding; cell structure disassembly during apoptosis

Disease: Cataract 30
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Research Articles on VIM

1. The VIM gene is frequently methylated in cervical squamous cell carcinoma and that its methylation might predict a favorable prognosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VIM recombinant protein

 Reference Product  PubMed Publications
 CASP3 recombinant protein  >8 publications with VIM and CASP3
 AKT1 recombinant protein  >4 publications with VIM and AKT1
 TPM3 recombinant protein  >1 publications with VIM and TPM3
 CASP8 recombinant protein  >1 publications with VIM and CASP8

Pathways associated with VIM recombinant protein

 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with VIM recombinant protein

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >185 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >68 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >45 publications with VIM and Kidney Diseases
 Disease Models, Animal Antibodies  >44 publications with VIM and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >43 publications with VIM and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >39 publications with VIM and Breast Neoplasms
 Liver Diseases Antibodies  >38 publications with VIM and Liver Diseases
 Fibrosis Antibodies  >38 publications with VIM and Fibrosis
 Necrosis Antibodies  >28 publications with VIM and Necrosis
 Neoplasm Invasiveness Antibodies  >26 publications with VIM and Neoplasm Invasiveness

Organs/Tissues associated with VIM recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >545 publications with VIM and Brain
 Blood Antibodies  >248 publications with VIM and Blood
 Muscle Antibodies  >132 publications with VIM and Muscle
 Bone Antibodies  >88 publications with VIM and Bone
 Nerve Antibodies  >88 publications with VIM and Nerve
 Kidney Antibodies  >69 publications with VIM and Kidney
 Liver Antibodies  >63 publications with VIM and Liver
 Bone Marrow Antibodies  >55 publications with VIM and Bone Marrow
 Eye Antibodies  >52 publications with VIM and Eye
 Lung Antibodies  >49 publications with VIM and Lung
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