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Calbindin (CALB1) Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS555685

Calbindin, also known as calbindin 1 or calbindin-D28k is a member of the large superfamily of cytoplasmic Ca2+ binding proteins. Calbindin-1 belongs to the subclass of these containing the "EF hand" Ca2+ binding motif originally characterized in parvalbumin.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Calbindin (CALB1), Monoclonal Antibody
Full Product Name
Calbindin, monoclonal antibody, mouse
Product Gene Name
Clonality
Monoclonal
Species Reactivity
Human, Horse, Cow, Pig, Chicken, Rat, Mouse
Form/Format
Liquid
Purity/Purification
Affinity Purified
Preparation and Storage
Antibody can also be aliquoted and stored frozen at -20°C -70°C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2-8°C for 1 month without detectable loss of activity. Avoid repeated freeze/thaw cycles.
OMIM
X06661 mRNA
3D Structure
ModBase 3D Structure for P05937
Immunogen Sequence
Full length recombinant human protein.
Applications Tested/Suitable for anti-CALB1 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CALB1 antibody
IF/ICC: 1:5,000
IHC: 1:5,000
WB: 1:5,000
Dilutions listed as a recommendation. Optimal dilution should be determined by the investigator.

Testing Data of anti-CALB1 antibody

Image: Adult mouse cortex (Left) and cerebellum (Right) sections were stained in red, and co-stained with our rabbit polyclonal anti-Fox3/NeuN antibody in green. Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). Fox3/NeuN expresses in most neurons; as a result, cells positive for calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.
Anti-CALB1 Monoclonal Antibody – Cat# MBS555685

Western Blot (WB) of anti-CALB1 antibody

Western blot analysis of MBS555685. Blots of cow cerebellum lysate were probed with MBS555685. The MBS555685 monoclonal binds strongly and cleanly to the calbindin band at 28 kDa
Anti-CALB1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS555685

Other Notes

Small volumes of anti-CALB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CALB1 purchase

MBS555685 is an antibody targeting CALB1. Also known as Calbindin, monoclonal antibody, mouse. Reactive in Human, Horse, Cow, Pig, Chicken, Rat, Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CALB1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calbindin
NCBI Official Synonym Full Names
calbindin 1
NCBI Official Symbol
CALB1
NCBI Official Synonym Symbols
CALB; D-28K
UniProt Protein Name
Calbindin
UniProt Synonym Protein Names
Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type
Protein Family
UniProt Gene Name
CALB1
UniProt Synonym Gene Names
CAB27
NCBI Protein Information
calbindin

NCBI Summary for CALB1

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
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UniProt Comments for CALB1

CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: axon; cell soma; cytosol; dendrite; nucleus; synapse

Molecular Function: calcium ion binding; protein binding; zinc ion binding

Biological Process: cellular protein metabolic process; cytosolic calcium ion homeostasis; long-term memory; short-term memory
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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