CCK-8 Antibody – Rabbit

Immunofluroescence: 1:100-1:200 (Fluorescein)
1:500-1:1,000 (Streptavidin/HRP).

Tissue Preparation:
10 um cryostats
Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30 min.
Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.

Immunohistochemistry:
The antibody has siginificant indirect immunofluorescent staining at a 1/100-1/200 dilution and significant Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1,000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10^-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: alphaACGRP, BETACGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide.
Optimal dilution should be determined by investigator.