Alpha-glia Expansion Culture Media

Microglial cells are the dominant antigen presenting cells in the central nervous system. Under resting conditions, the expression of the molecular complex for presenting antigen, the major histocompatibility complex II (MHCII) and co-stimulatory molecules such as CD80, CD86 and CD40 is below detection. Upon injury the molecules are highly upregulated and the expression of this complex is essential for interacting with T lymphocytes. This upregulation has been described in a number of pathologies and is well studied in Multiple Sclerosis. Microglial cells phagocytose myelin, degrade it and present peptides of the myelin proteins as antigens. By releasing cytokines such as CCl2 microglial cells are important for recruiting leucocytes into the CNS. Microglia interact with infiltrating T lymphocytes and, thus, mediate the immune response in the brain. They have the capacity to stimulate proliferation of both TH1- and TH2-CD4 positive T cells.
Alpha-glia Expansion Medium is a complete medium designed to promote optimal growth of normal human brain-derived microglia in vitro. It is a sterile, liquid medium which contains:
Essential and non-essential amino acids
Vitamins
Organic and inorganic compounds
Hormones
Growth factors
Trace minerals
Fetal bovine serum (5%).
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human microglia in vitro. Alpha-glia Expansion Medium consists of 445 ml of basal medium, 25 ml of fetal bovine serum 25 ml of microglia growth supplement and 5 ml of penicillin/streptomycin solution.

Small volumes of Alpha-glia Expansion culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS555782 is a culture media targeting Alpha-glia Expansion. Also known as Alpha-glia Expansion Medium. Reactive in Human.